Response of haemolymph oxygen affinity to simultaneous salinity and oxygen stress in the intertidal prawn, Palaemon elegans (rathke)

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Abstract

  • 1.

    1. Specimens of the intertidal prawn Palaemon elegans were acclimated to nine different combinations of salinity and oxygen availability. The conditions ranged from hyposaline (15%o) to hypersaline (40%o) and from hypoxia (50 Torr) to hyperoxia (500 Torr).

  • 2.

    2. P. elegans was able to regulate haemolymph Ca2+, Mg2+ and Cl, independent of the oxygen tension of the water.

  • 3.

    3. The oxygen affinity and pH sensitivity of oxygen binding by the haemocyanin both increased when the acclimation salinity was highest at 40%, but were unaffected by the acclimation oxygen tension.

  • 4.

    4. The dependence of oxygen affinity on the concentration of l-lactate was relatively low (Alog P50/Alog[lac] ranged from −0.10 to −0.23) but was independent of the acclimation salinity and oxygen tension.

  • 5.

    5. It was concluded that acclimation to the conditions used influenced oxygen affinity primarily via altered concentrations of Ca2+ and Mg2+ in the haemolymph.

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