Elsevier

Gene

Volume 95, Issue 1, 30 October 1990, Pages 133-135
Gene

Construction of a promoter-probe shuttle vector for Escherichia coli and brevibacteria

https://doi.org/10.1016/0378-1119(90)90424-PGet rights and content

Abstract

We constructed a promoter-probe vector, pJUP05, for brevibacteria and Escherichia coli based on the promoterless neomycin-resistance (neoR) gene from Tn5. This gene confers resistance to the aminoglycosides, kanamycin and neomycin. The promoter of the neoR gene was deleted and replaced by a suitable multiple cloning site. There are translation stop codons in all three reading frames upstream from the neoR gene. The plasmid contains functional origins of DNA replication for both brevibacteria and E. coli, and permits selection for chloramphenicol- and/or ampicillin-resistance markers.

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