Elsevier

Gene

Volume 116, Issue 1, 1 July 1992, Pages 105-108
Gene

Cloning and sequence analysis of a Candida maltosa gene which confers resistance to cycloheximide

https://doi.org/10.1016/0378-1119(92)90636-4Get rights and content

Abstract

A CYHR gene from Candida maltosa, which confers resistance to cycloheximide, was cloned in Saccharomyces cerevisiae. A 2.3-kb DNA fragment carrying this gene was sequenced, and an open reading frame able to encode 553 amino acids (aa) was found in the sequence. Computer searches of the GenBank, EMBL, SWIS-PROT and Gen-Pept databases using the FASTA program failed to detect any proteins with extensive similarities to the deduced aa sequence for CYHR. The cloned gene transforms S. cerevisiae at a frequency similar to auxotrophic markers and can be used as a dominant selectable marker for introducing recombinant plasmids into wild-type strains of S. cerevisiae, as well as for gene disruption experiments.

References (13)

There are more references available in the full text version of this article.

Cited by (40)

  • Limited compatibility of polymerase subunit interactions in influenza A and B viruses

    2010, Journal of Biological Chemistry
    Citation Excerpt :

    Yeast Saccharomyces cerevisiae 214Δpep4 MATa (ura3 leu2 his3 Δpep4) was used for the expression of PA proteins. Transformation and subsequent selection were carried out as described (27). For protein expression, yeast cells were inoculated into selective YEPD and grown for 24 h at 28 °C; thereafter yeast cells were reinoculated into YEPG induction medium and cultured further for 24 h at 28 °C.

  • A Gcn4p homolog is essential for the induction of a ribosomal protein L41 variant responsible for cycloheximide resistance in the yeast Candida maltosa

    2004, Journal of Biological Chemistry
    Citation Excerpt :

    This leakiness of inhibition by CYH is probably brought about by another CYH resistance mechanism because a C. maltosa mutant that lacks all the functional L41-Q genes, L41-Q2a, L41-Q2b, and L41-Q3 (L41-Q3 has no allele), was able to slowly grow on 25 μg/ml CYH.3 This resistance is at least in part due to a homolog to C. albicans CaMDR1, which gave high CYH resistance to S. cerevisiae (57).4 The stabilization of C-GCN4 mRNA by CYH could have also helped the induction of L41-Q.

  • Drug resistance in yeasts - An emerging scenario

    2002, Advances in Microbial Physiology
  • Novel target genes of the yeast regulator Pdr1p: A contribution of the TPO1 gene in resistance to quinidine and other drugs

    2001, Gene
    Citation Excerpt :

    Related but nevertheless distinct fungal homologues of Tpo1p include the cycloheximide resistance protein CyhR (TC 2. A.1.2.2) of Candida maltosa (Sasnauskas et al., 1992) and the CaMDR1 protein (TC 2. A.1.2.6) of Candida albicans, which has been found to be overexpressed in drug-resistant clinical isolates of this pathogenic yeast (Sanglard et al., 1995).

View all citing articles on Scopus
View full text