Cloning and sequence analysis of a Candida maltosa gene which confers resistance to cycloheximide
References (13)
- et al.
Codon selection in yeast
J. Biol. Chem.
(1982) - et al.
Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast
Cell
(1982) - et al.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Analyt. Biochem.
(1983) - et al.
The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation
Gene
(1984) - et al.
Molecular cloning of the Candida maltosa ADE1 gene
Gene
(1991) - et al.
Transformation of intact yeast cells treated with alkali cations
J. Bacteriol.
(1983)
Cited by (40)
The efflux pumps in medical mycology: Molecular mechanisms and therapeutic perspectives
2010, Journal de Mycologie MedicaleLimited compatibility of polymerase subunit interactions in influenza A and B viruses
2010, Journal of Biological ChemistryCitation Excerpt :Yeast Saccharomyces cerevisiae 214Δpep4 MATa (ura3 leu2 his3 Δpep4) was used for the expression of PA proteins. Transformation and subsequent selection were carried out as described (27). For protein expression, yeast cells were inoculated into selective YEPD and grown for 24 h at 28 °C; thereafter yeast cells were reinoculated into YEPG induction medium and cultured further for 24 h at 28 °C.
A Gcn4p homolog is essential for the induction of a ribosomal protein L41 variant responsible for cycloheximide resistance in the yeast Candida maltosa
2004, Journal of Biological ChemistryCitation Excerpt :This leakiness of inhibition by CYH is probably brought about by another CYH resistance mechanism because a C. maltosa mutant that lacks all the functional L41-Q genes, L41-Q2a, L41-Q2b, and L41-Q3 (L41-Q3 has no allele), was able to slowly grow on 25 μg/ml CYH.3 This resistance is at least in part due to a homolog to C. albicans CaMDR1, which gave high CYH resistance to S. cerevisiae (57).4 The stabilization of C-GCN4 mRNA by CYH could have also helped the induction of L41-Q.
Drug resistance in yeasts - An emerging scenario
2002, Advances in Microbial PhysiologyNovel target genes of the yeast regulator Pdr1p: A contribution of the TPO1 gene in resistance to quinidine and other drugs
2001, GeneCitation Excerpt :Related but nevertheless distinct fungal homologues of Tpo1p include the cycloheximide resistance protein CyhR (TC 2. A.1.2.2) of Candida maltosa (Sasnauskas et al., 1992) and the CaMDR1 protein (TC 2. A.1.2.6) of Candida albicans, which has been found to be overexpressed in drug-resistant clinical isolates of this pathogenic yeast (Sanglard et al., 1995).