Effect of insulin on the incorporation of [13C]leucine into rat caudofemoralis protein

https://doi.org/10.1016/0304-4165(67)90105-5Get rights and content

Abstract

This study sought first to develop in in vitro preparation representative of peripheral skeletal muscle and then to study the effects of insulin on the incorporation of labelled amino acid into varous protein fractions thereof. Rat caudofemoralis muscle proteins were separated on the basis of their solubility in solutions of increasing ionic strenght and pH. The specific radioactivity of various protein fractions was found to be inversely proportional to the ionic and alkaline strength of the extracting solution. Insulin stimulated incorporation of [14C]leucine into each fraction with approximately equal magnitude. Likewise, the distribution (ratio) of counts in several protein fractions eluted from a DEAE-cellulose column was not materially affected by insulin. Our results fit the hypothesis that insulin stimulates the incorporation of [14C]leucine into skeletal muscle protein by increasing its entry into cells and not by stimulation of synthesis of one or more specific proteins.

References (39)

  • D.M. Kipnis et al.

    Biochem. Biophys. Acta

    (1958)
  • I.G. Wool
  • O.H. Lowry et al.

    J. Biol. Chem.

    (1951)
  • R.R. Porter
  • M.E. Krahl

    J. Biol. Chem.

    (1953)
  • F.M. Sinex et al.

    J. Biol. Chem.

    (1952)
  • D.M. Kipnis et al.

    J. Biol. Chem.

    (1957)
  • J.J. Castles et al.

    Biochem. J.

    (1964)
  • D.M. Kipnis et al.
  • K.L. Machester et al.

    J. Biol. Chem.

    (1959)
  • I.G. Wool et al.

    Nature

    (1959)
  • R. Scharff et al.

    Biochem. J.

    (1956)
  • R. Scharff et al.

    Biochem. J.

    (1956)
  • I.G. Wool
  • P.P. Cohen
  • E.N. Sassenrath et al.

    Cancer Res.

    (1954)
  • D.S. Robinson

    Biochem. J.

    (1952)
  • A. Szent-Györgi
  • M.E. Krahl

    Science

    (1952)
  • Cited by (0)

    Present address: Department of Bacteriology and Immunology, Harvard Medical School, Boston, Mass. 02115, U.S.A.

    View full text