N-acetylaspartic acid in G-actin

doi:10.1016/0304-4165(66)90164-4

Biochimica et Biophysica Acta (BBA) - General Subjects

Volume 117, Issue 1, 28 March 1966, Pages 176-183

https://doi.org/10.1016/0304-4165(66)90164-4 Get rights and content

Abstract

A ninhydrin-negative peptide fraction obtained from pronase digestion of G-actin was isolated on a Dowex-50 X-2 (H⁺) column. Analysis of this fraction by gas chromatography showed that most of the acetyl content of the original actin was recovered. The presence of an acetyl group in the peptide fraction was also demonstrated by hydrazinolysis. This fraction consists mainly of a tripeptide which, upon degradation with carboxypeptidase A (EC 3.4.2.1, had an amino acid sequence of N-acetyl-Asp-Glu-Thr. It is suggested that N-acetylaspartic acid is the NH₂-terminal residue of G-actin.

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N-acetylaspartic acid in G-actin

Abstract

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Biochim. Biophys. Acta

J. Biol. Chem.

J. Biol. Chem.

Biochem. J.

J. Biochem. Tokyo