Lipid-oligosaccharide intermediate of glycoprotein synthesis in Physarum polycephalum

doi:10.1016/0304-4165(86)90108-X

Biochimica et Biophysica Acta (BBA) - General Subjects

Volume 881, Issue 1, 19 March 1986, Pages 148-153

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Abstract

Lipid-linked oligosaccharides were extracted from microplasmodia of Physarum polycephalum grown in the presence of [³H]mannose, [¹⁴C]glucose of $N- acetyl [^{3} H] glucosamine$ . The lipid-oligosaccharide conjugate was purified by column cromatography with DEAE-Sephacel and the oligosaccharide moiety was released by acid hydrolysis. The oligosaccharides were treated with α-mannnosidase and endoglucosaminidase H and the resulting products were analyzed. The results suggest that the lipid-linked oligosaccharide of P. polycephalum is similar, if not identical, to that reported for the lipid-oligosaccharide intermediate in glycoprotein synthesis in animal and plant cells. The biosyenthesis of this oligosaccharide was greatly depressed when the plasmodia underwent differentiation to macrocyts.

References (16)

J.W. Daniel et al.
O.H. Lowry et al.
J. Biol. Chem.
(1951)
T. Nobori et al.
Exp. Cell Res.
(1984)
H. Hori et al.
Arch. Biochem. Biophys.
(1982)
J. Chambers et al.
J. Biol. Chem.
(1975)
R.J. Chalifour et al.
Arch. Biochem. Biophys.
(1984)
K. Zaar et al.
Biochim. Biophys. Acta
(1979)
R.G. Spiro et al.
J. Biol. Chem.
(1976)

There are more references available in the full text version of this article.

Cited by (4)

Study of the N-glycoprotein biosynthesis through dolichol intermediates in the mitochondrial membranes
1989, International Journal of Biochemistry
- 1.
  1. In the mitochondria, the biosynthesis of N-glycoprotein products, through the dolichol intermediates pathway, appears in the outer and in the inner membranes.
- 2.
  2. The biosynthesis of dolichol-pyrophosphoryl-N-acetyl-glucosamine, dolichol-pyrophosphoryl-di-N-acetylchitobiose, dolichol-phosphoryl-glucose and dolichol-phosphoryl-mannose is effective in both membranes.
- 3.
  3. The lipid-linked oligosaccharides biosynthesized in both membranes contain high mannose-type oligosaccharides ranging in size from Man₉-GlcNac₂ to Man₄-GlcNac₂.
- 4.
  4. The assembly of the dolichol-pyrophosphoryl-oligosaccharides on the trimannosidic core begins by the elongation of the α-1,3 mannose branch in the outer membrane and of the α-1,6 mannose branch in the inner membrane.
Changes in membrane glycoproteins during fruit-body formation in Physarum polycephalum
1992, The Botanical Magazine Tokyo
Glycoproteins and enzymes associated with the plasmodial membrane and slime layer of physarum polycephalum
1991, Journal of General and Applied Microbiology
Characterization of glycopeptides from physarum polycephalum labeled with [<sup>3</sup>H]mannose or [<sup>3</sup>H]glucosamine
1989, The Journal of General and Applied Microbiology

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Lipid-oligosaccharide intermediate of glycoprotein synthesis in Physarum polycephalum

Abstract

J. Biol. Chem.

Exp. Cell Res.

Arch. Biochem. Biophys.

J. Biol. Chem.

Arch. Biochem. Biophys.

Biochim. Biophys. Acta

J. Biol. Chem.