Characterization of pseudo-islets formed from pancreatic islet cell suspensions of neonatal rats

https://doi.org/10.1016/0303-7207(83)90081-3Get rights and content

Abstract

Well-preserved pancreatic islet cell suspensions were prepared from islets of Langerhans of neonatal rats by gentle trypsin treatment. Within a culture period of 4–6 days the islet cells reaggregate spontaneously and form pseudo-islets of different size and of a variable insulin content. While the ratio of insulin to glucagon in isolated islets of Langerhans is constant (18 ± 1.9), the hormone ratio of the pseudo-islets is strongly variable and increased, indicating an excess of insulin. Glucose enhancement from 1.5 mmoles/l to 15 mmoles/1 results in a significant stimulation of (pro)insulin biosynthesis whereas insulin secretion of the pseudo-islets is only slightly increased. At high glucose concentration (15 mmoles/1) insulin secretion of the pseudo-islets can be potentiated (by a factor of 4.5 ±0.46) by 3-isobutyl-1-methylxanthine (IBMX). Compared with the initial islet cell suspension, the cell aggregation during pseudo-islet formation did not result in an enhanced secretory response on glucose stimulation.

References (36)

  • M.H. Greider et al.
  • P. Meda et al.

    Exp. Cell. Res.

    (1981)
  • A. Niki et al.

    FEBS Lett.

    (1977)
  • L.D. Britt et al.

    Diabetes

    (1981)
  • D. Gottschling et al.

    Radiobiol. Radiother.

    (1974)
  • P.A. Halban et al.

    Endocrinology

    (1982)
  • B. Hehmke et al.

    Acta Biol. Med. Germ.

    (1982)
  • B. Hillman et al.
  • I. Hilwig et al.

    Z. Zellforsch.

    (1970)
  • L.A. Idahl et al.

    Pflügers Arch.

    (1976)
  • H. Jahr et al.

    Eur. J. Biochem.

    (1980)
  • T. Kanatsuna et al.

    Clin. Exp. Immunol.

    (1982)
  • E. Kohen et al.

    Science

    (1979)
  • U. Krause et al.

    Hormone Metab. Res.

    (1973)
  • H. Kromann et al.

    Med. Biol.

    (1980)
  • Å. Lemmark

    Diabetologia

    (1974)
  • Å. Lernmark
  • A. Lernmark et al.

    J. Cell. Biol.

    (1976)
  • Cited by (9)

    View all citing articles on Scopus

    Part of this work was presented at the 3rd International Workshop “Isolated pancreatic islets and diabetes research” held at Heringsdorf (G.D.R.), May 1982.

    View full text