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Simultaneous effect of gonadotropin-releasing hormone (GnRH) on the expression of two gonadotropin β genes by passive immunization to GnRH

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Abstract

In order to reveal the action of gonadotropin-releasing hormone (GnRH) on the synthesis of gonadotropins in the pituitary gland of castrated rats, passive immunization to GnRH designed to block the activity of GnRH was performed. The levels of prolactin mRNA in castrated and rabbit anti-GnRH serum (RAGnRH)-treated rats decreased, whereas TSHß mRNA showed no statistically significant change. In contrast, mRNAs encoding common α, LHß and FSHß were increased 2.7- 1.7- and 1.5-fold, respectively, by castration. These elevated mRNA levels of gonadotropin subunits in castrated rats well explain the increased hormone levels in serum and in the pituitary. Two days later, a single administration of RAGnRH to the castrated rats significantly suppressed the mRNA levels to 2.0-fold for a 1.2-fold for LHß and 1.1-fold for FSHß relative to the respective control values. These results showed that the two gonadotropin β genes respond more rapidly to GnRH action than the common a gene.

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