Complexes of aspartate aminotransferase with hydroxylamine derivatives: spectral studies in solution and in the crystalline state

Abstract

Hydroxylamine and its derivatives of general formula H₂NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the α-carboxylate group. Oximes formed between such compounds and pyridoxal phosphate in the active site of aspartate amino-transferase mimic external aldimine intermediates that occur during catalys is by this enzyme. The properties of oxime derivatives of mitochodrial aspartate aminotransferase with hydroxylamine and 6 compounds H₂NOR were studied by absorption spectroscopy and circular dichroism in solution and by linear dichroism irystals. Stable oximes, absorbing at λ_max ⋍ 380 nm and exhibiting a negative Cotton effect, were obtained with the carboxylate-containing compounds. The oximes formed with carboxylate-free compounds showed somewhat different properties and stability. With H-Tyr a stable complex absorbing at λ_max ⋍ 370 nm rather than at 380 nm, was obtained, H-Ala and H-Phe produced unstable oximes with the initial absorption band at λ_max ⋍ 380 nm that was gradually replaced by a band at λ_max ⋍ 340 nm. The species absorbing at 340 nm were shown to be coenzyme-inhibitor complexes which were gradually released from the enzyme. A similar 330–340 nm absorption band was observed upon reaction of the free coenzyme with all hydroxylamine inhibitors at neutral pH-values. The results of the circular dichroism experiments in solution and the linear dichroism studies in microcrystals of mAspAT indicate that the coenzyme conformation in these inhibitor / enzyme complexes is similar to that occurring in an external aldimine analogue, the 2-MeAsp / mAspAT complex. Co-crystallizations of the enzyme with the H₂NOR compounds were also carried out. Triclinic crystals were obtained in all cases, suggesting that the “closed” structure cannot be stabilized by a single carboxylate group.