Lipoxygenases from Zea mays L. Purification and physicochemical characteristics

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Abstract

Maize (Zea mays L.) seeds after 5 d germination contain at least two lipoxygenase isoenzymes, L1 and L2. Enzymes were extracted from acetone powder with 0.1 M sodium acetate (pH 4.5) buffer, supplemented with a non-ionic detergent (0.1% Brij 99) and DETAPAC (0.1 mM). The pI of L1 is 6.40 and isoelectric focusing of L2 results in two peaks with pI values in close proximity: 5.55 and 5.70. L1 and L2 are monomeric proteins of Mr 100000 and 90000, respectively. Linoleic acid, 18:2(n - 6), is a good substrate for L2, but L1 has more affinity for α-linolenic acid, 18:3(n − 3). These kinetic studies could indicate a different functional role of the two isoenzymes.

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