Fluoroscence studies of chloropyll α incorporated into lipid mixtures, and the interpretation of “phase” diagrams

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Abstract

The fluorescence of chloropyll α incorporated into liposomes of mixtures of phosphatidylcholines and phosphatidylethanolamines is reprted. Plots of fluorescence intensities against temperature show breaks at characteristic temperatures which can be attributed to the onset and completion of solid phase lipid formation. These temperatures can be plotted to give diagrams analogous to the phase diagrams obtained for macroscopic systems. Complications due to “small-system effects” are discussed, and the experimental diagrams are compared with theoretical phase digrams calculated for ideal mixing. Introduction of cholesterol leads to a reduction in fluorescence intensity, most readily explained by a1 : 1 lipid :cholesterol interaction with exclution of monomeric, fluorescent, chlorophyll a. Interaction of divalent ions with mixtures of dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylserine leads to exclution oc chlorophyll a from the phosphatidylserine.

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