Elsevier

Carbohydrate Research

Volume 59, Issue 1, November 1977, Pages 165-177
Carbohydrate Research

Klebsiella serotype-13 capsular polysaccharide: Primary structure and depolymerization by a bacteriophage-borne glycanase

https://doi.org/10.1016/S0008-6215(00)83303-0Get rights and content

Abstract

Periodate oxidation and Smith degradation, methylation analysis including uronic acid degradation, partial hydrolysis with acid, bacteriophage degradation, and p.m.r. spectroscopy have been used to elucidate the primary structure of the Klebsiella serotype-13 capsular polysaccharide. The polymer consists of pentasaccharide repeating-units comprising a 4)-β-D-Man p-(1 → 4)-α-D-Glcp-(1 → 3)-β-D-Glcp-(1 → chain with a 3,4-O-(1-car☐yethylidene)-β-D-Gal p-(1 → 4)-α-D-GlcAp(1 → branch at position 3 of the mannose. It is shown that there is a glycanase activity associated with particles of Klebsiella bacteriophage No. 13. which catalyses hydrolysis of chain β-D-Glcp-(1 → 4)-β-D-Man p linkages in the type-13 polysaccharide. The chemical basis of some serological cross-reactions of the Klebsiella K13 antigen is discussed.

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