Regular Article
Protein-Binding DNA Regions inside and in the Vicinity of the Cytochrome P450bm-3 Gene from Bacillus megaterium

https://doi.org/10.1006/bbrc.1994.1123Get rights and content

Abstract

Protein-binding DNA-regions inside and in the vicinity of the phenobarbital-inducible P450bm-3 gene from Bacillus megaterium were characterized by gel-retardation technique and foot-printing analysis. Regions with induction-dependent protein binding capacity were shown to be situated in −279bp/−215bp, −215bp/+83bp and +236bp/+425bp fragments. Precise localization of DNA-protein contacts was established by foot-printing analysis for region −215bp/+83bp. The data obtained are discussed in line with previously reported information on the regulatory regions of various phenobarbital-regulated genes.

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