Inhibition of phorbol ester-caused induction of ornithine decarboxylase and tumor promotion in mouse skin by staurosporine, a potent inhibitor of protein kinase C

doi:10.1016/S0006-291X(88)80003-2

Biochemical and Biophysical Research Communications

Volume 157, Issue 1, 30 November 1988, Pages 9-15

https://doi.org/10.1016/S0006-291X(88)80003-2 Get rights and content

Summary

We found that staurosporine, a potent inhibitor of protein kinase C, inhibits induction of ornithine decarboxylase (ODC) and tumor promotion caused by 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD-1 mouse skin. When applied 5 min either before or after treatment with TPA, 1 μg of staurosporine caused about 56% inhibition of ODC-induction by 5 μg of TPA. However, staurosporine did not inhibit TPA-induced epidermal hyperplasia. In two-stage carcinogenesis, staurosporine at 1 μg was applied 5 min before application of 5 μg of TPA to the initiated skin: number of tumors was suppresed by about 40% although the incidence was not affected. No tumors developed when staurosporine alone was applied to the initiated skin.

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^*: Supported in part by a Grant-in-Aid for Special Project Research, Cancer-Bioscience, from the Ministry of Education, Science and Culture of Japan.

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Inhibition of phorbol ester-caused induction of ornithine decarboxylase and tumor promotion in mouse skin by staurosporine, a potent inhibitor of protein kinase C*

Summary

J. Biol. Chem.

Biochem. Biophys. Res. Commun.

Anal. Biochem.

Biochem. Biophys. Res. Commun.

Biochemical Pharmacol.

Biochem. Biophys. Res. Commun.

Biochem. Biophys. Res. Commun.

Nature