Effects of CPTA upon carotenogenesis and lipoidal constituents in Rhodotorula species

doi:10.1016/0031-9422(74)80085-3

Phytochemistry

Volume 13, Issue 7, July 1974, Pages 1123-1127

https://doi.org/10.1016/0031-9422(74)80085-3 Get rights and content

Abstract

The effects of 2-(4-chlorophenyl)-triethylamine (CPTA) upon carotenogenesis in Rhodotorula glutinis, and upon various lipoidal constituents of R. rubra were studied. CPTA was found to cause the accumulation of lycopene and γ-carotene and to inhibit the formation of fatty acids and ergosterol. Upon removal of the inhibitor lycopene was metabolized to the cyclic carotenes and the levels of ergosterol increased. It was proposed that CPTA inhibits the cyclase enzyme of carotenogenesis, inhibits the formation of ergosterol, and causes the build-up of intermediates of these compounds.

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Cited by (6)

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Carotenoid biosynthesis was examined in a phylloplane yeast identified by ITS, 18S and 28S rDNA analysis as a Dioszegia sp. close to D. takashimae. In well-aerated flask or fermentor cultures, this strain produced essentially a single pigment confirmed as the xanthophyll plectaniaxanthin by NMR analysis, at concentrations of 103–175 μg g⁻¹ biomass dry weight. Detailed studies showed increases in plectaniaxanthin concentrations in the presence of 5 mM hydrogen peroxide (1.8-fold), 50 and 100 μM duroquinone (3.1- and 3.7-fold, respectively), and 2% ethanol (4.9-fold). Whereas oxidative stress is known to enhance the biosynthesis of torularhodin or astaxanthin in other red yeasts where they are associated with an antioxidant function, this is the first report implicating plectaniaxanthin in a similar role. At reduced aeration, biosynthesis of plectaniaxanthin was suppressed and its putative precursor γ-carotene accumulated. The carotenoid cyclase inhibitor nicotine (5–20 mM) inhibited plectaniaxanthin formation, with lycopene accumulating stoichiometrically. Hydroxy groups at C-1′ and C-2′ therefore seem to be introduced late in plectaniaxanthin biosynthesis, following cyclization of the β-ionone ring.
Effect of CPTA on carotenogenesis by Phycomyces carA mutants
1980, Plant Science Letters
The presence in the medium of 2-(4-chlorophenylthio)-triethylamine hydrochloride (CPTA), an inhibitor of carotenogenesis, results in an increase of carotenoids. Lycopene and γ-carotene are the main carotenoids form no carotenoids. Lycopene and γ-carotene are the main carotenoids accumulated. The effect of CPTA on carA mutants does not occur in the presence of the protein-synthesis inhibitor cycloheximide. These and other results, previously reported, suggest that blocking of lycopene cyclisation by CPTA releases a negative control by β-carotene and that gene carA is involved in this regulatory mechanism. Probably the effect of carA mutations is an extreme reduction of the basal level of β-carotene required to trigger the negative control.
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1986, Critical Reviews in Plant Sciences
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1977, Journal of Agricultural and Food Chemistry
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1977, Journal of Agricultural and Food Chemistry
Later reactions of carotenoid biosynthesis
1976, Pure and Applied Chemistry

^☆: Contribution No. 1515 of the Rhode Island Agricultural Experiment Station.

¹: Present address: Fruit and Vegetable Chemistry Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Pasadena, CA 91106, U.S.A.

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Phytochemistry

Effects of CPTA upon carotenogenesis and lipoidal constituents in Rhodotorula species☆

Abstract

Phytochemistry

Phytochemistry

Phytochemistry

Phytochemistry

Phytochemistry

Phytochemistry

Biosynthesis of the xanthophyll plectaniaxanthin as a stress response in the red yeast Dioszegia (Tremellales, Heterobasidiomycetes, Fungi)

Effect of CPTA on carotenogenesis by Phycomyces carA mutants

Biosynthesis of carotenes in higher plants

Carotenoid Induction in Orange Endocarp

Effect of High Temperature on CPTA-Induced Carotenoid Biosynthesis in Ripening Tomato Fruits

Later reactions of carotenoid biosynthesis