Subunit separation in reversed micelle system reveals the existence of active centers both on light and heavy γ-glutamyltransferase subunits
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Keywords
γ-Glutamyltransferase
Protein subunit
Micellar enzymology
Reversed micelle
Supra-macromolecular structure
Abbreviations
γ-GT
γ-glutamyltransferase
AOT
Aerosol OT (sodium bis(2-ethylhexyl)sulfosuccinate)
NA
(4-nitro)aniline
CNA
(3-carboxy-4-nitro)aniline
GluNA
γ-glutamyl(4-nitro)anilide
GluCNA
γ-glutamyl(3-carboxy-4-nitro)anilide
AT-125
L-(αS,5S)-α-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid
GlyGly
glycylglycine
RM
reversed micelle
PAGE
polyacrylamide gel electrophoresis
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Copyright © 1991 Published by Elsevier B.V.