Elsevier

FEBS Letters

Volume 174, Issue 2, 3 September 1984, Pages 248-252
FEBS Letters

Research letters
Phosphorus-31 nuclear magnetic resonance studies of photosynthesizing Chlorella

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Abstract

Phosphorus-31 nuclear magnetic resonance studies of intact Chlorella cells under light and dark conditions are described Pi, ATP, NAD, UDP-glucose and polyphosphate were observed in the cell The presence of two compartments was postulated from two intracellular P1 signals, whose chemical shift values were depen- dent on illumination These two pi signals were assigned to those in the stroma of chloroplasts and in the cytoplasm based on their response to the light and dark cycle, and to the treatment of cells with 3-(3,4- dichlorophenyl)-1,1-dimethylurea In the light the chloroplastic pH became more alkaline, while the cyto- plasmic pH became more acidic An increase in ATP was also observed upon illumination

31P-NMR Chlorella Intracellular pH Chloroplast Cytoplasm Photophosphorylation

Abbreviations

CCCP
carbonyl cyanide m-chlorophenyl hydrazone
DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea
MDP
methylene diphosphonic acid

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