Elsevier

FEBS Letters

Volume 261, Issue 1, 12 February 1990, Pages 85-88
FEBS Letters

Research letters
Members of the α-amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

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Abstract

We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the α-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.

Keywords

Allergen
Baker's asthma
α-Amylase inhibitor
(wheat)

Abbreviations

IEF, isoelectrofocusing
PVDF, polyvinyldene difluoride
RAST, radioallergosorbent test
SDS-PAGE, SDS-polyacrylamide gel electrophoresis
SGE, starch gel electrophoresis
WDAI-3, wheat dimeric α-amylase inhibitor-3

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