The turnover number for band 3-mediated sulfate transport in phosphatidylcholine bilayers

doi:10.1016/0014-5793(88)81407-8

FEBS Letters

Volume 227, Issue 1, 18 January 1988, Pages 32-34

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Abstract

The anion transport system of the human erythrocyte membrane was reconstituted in unilamellar phosphatidylcholine vesicles, and a vesicle subpopulation of a narrow size distribution was isolated from the sample by gel filtration. In this subpopulation, the turnover number of the transport protein (the band 3 protein) for sulfate transport was determined. It was found that, in the reconstituted system, the protein transports sulfate 5–10-times faster than in the human erythrocyte membrane.

Keywords

Band 3 protein

Sulfate transport

Reconstitution

Turnover number

(Erythrocyte membrane)

Abbreviations

PC, phosphatidylcholine

H₂DIDS, 4,4′-diisothiocyanostilbene-2,2′-disulfonate