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Crystals of human recombinant N-acetylgalactosamine-4-sulfatase have been grown using vapour diffusion. The protein contains approximately 13%(w/w) carbohydrate. The crystals belong to the tetragonal space group P41212 or its enantiomorph P43212 with a = b = 108.0 and c = 145.5 Å. The crystals diffract to 2.7 Å resolution.