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Liquid chromatographic analysis of clenbuterol residues in food products of animal origin (cow liver) using diphasic dialysis extraction

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This paper reports a method employing diphasic dialysis extraction for the rapid confirmation of clenbuterol in cow liver by RPHPLC with diode-array detection. The most suitable means of homogenizing the liver is in barium hydroxide-barium chloride buffer; the optimum extraction solvent istert-butyl methyl ether at an extraction temperature of 37°C; stirring should be applied at 150 r.p.m. for 4h. This extraction method improves clenbuterol recovery values to 98.9%.

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Authors and Affiliations

  1. Laboratorio de Salud Pública de Lugo, Consellería de Sanidade e Benestar Social, Xunta de Galicia, Lugo, Spain

    P. González Gigosos

  2. Laboratorio de Higiene e Inspección de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Veterinaria, Campus de Lugo, Universidad de Santiago de Compostela, Spain

    C. A. Fente Sampayo, C. Franco Abuín, B. Vázquez Belda, E. Quinto Fernández & A. Cepeda Sáez

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  1. P. González Gigosos
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  2. C. A. Fente Sampayo
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  3. C. Franco Abuín
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  4. B. Vázquez Belda
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  5. E. Quinto Fernández
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  6. A. Cepeda Sáez
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González Gigosos, P., Fente Sampayo, C.A., Franco Abuín, C. et al. Liquid chromatographic analysis of clenbuterol residues in food products of animal origin (cow liver) using diphasic dialysis extraction. Chromatographia 43, 271–274 (1996). https://doi.org/10.1007/BF02270993

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  • DOI: https://doi.org/10.1007/BF02270993

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