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Influence of solvent effects on retention in reversed-phase liquid chromatography and solid-phase extraction using a cyanopropylsiloxane-bonded, silica-based sorbent

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Summary

The solvation parameter model is used to characterize the retention properties of a cyanopropylsiloxane-bonded, silica-based sorbent with methanol, acetonitrile, tetrahydrofuran, and isopropanol in water as mobile phases. The system constants over the composition range 1 to 50% (v/v) organic solvent indicate that retention occurs because of the relative ease of cavity formation in the solvated stationary phase compared to the same process in the predominantly aqueous mobile phase as well as from more favorable stationary phase interactions with solutes containing π- and n-electrons. The capacity of the solute for dipole-type interactions is not important whereas all hydrogen-bond-type interactions result in reduced retention. Graphing the system constants as a function of mobile phase composition provides a simple mechanism for interpreting the change in capacity of the chromatographic system for retention in terms of changes in the relative weighting of fundamental intermolecular interactions. A comparison is also made with the retention properties of an octadecylsiloxane-bonded, silica-based sorbent with 30% (v/v) methanol in water as the mobile phase and the extraction characteristics of a porous polymer sorbent with 1% (v/v) methanol, acetonitrile, tetrahydrofuran, and isopropanol in water as the sample processing solvent. Changes in sorbent selectivity due to selective uptake of the processing solvent are much smaller for the cyanopropylsiloxane-bonded sorbent than the results found for a porous polymer sorbent.

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References

  1. C. F. Poole, S. K. Poole, “Chromatography Today”, Elsevier, Amsterdam, 1991, p. 394–402.

    Google Scholar 

  2. J. G. Dorsey, K. A. Dill, Chem. Rev.89, 331 (1989).

    Google Scholar 

  3. M. Jaroniec, J. Chromatogr. A656, 37 (1993).

    Google Scholar 

  4. K. B. Sentell, J. Chromatogr. A656, 231 (1993).

    Google Scholar 

  5. J. G. Dorsey, W. T. Cooper, Anal. Chem.66, 857A (1994).

    Google Scholar 

  6. C. F. Poole, S. K. Poole, “Chromatography Today”, Elsevier, Amsterdam, 1991, p. 469.

    Google Scholar 

  7. J. C. Berridge, “Techniques for the Automated Optimization of HPLC Separations”, Wiley, New York, NY, 1985.

    Google Scholar 

  8. L. R. Snyder, J. L. Glajch, J. J. Kirkland, “Practical HPLC Methods Development”, Wiley, New York, NY, 1988.

    Google Scholar 

  9. M. H. Abraham, Chem. Soc. Rev.48, 4 (1993).

    Google Scholar 

  10. M. H. Abraham, G. S. Whiting, R. M. Doherty, W. J. Shuely, J. Chem. Soc. Trans.2, 1451 (1990).

    Google Scholar 

  11. M. H. Abraham, G. S. Whiting, R. M. Doherty, W. J. Shuely, J. Chromatogr.518, 329 (1990).

    Google Scholar 

  12. M. H. Abraham, G. S. Whiting, R. M. Doherty, W. J. Shuely, J. Chromatogr.587, 229 (1991).

    Google Scholar 

  13. C. F. Poole, T. O. Kollie, S. K. Poole, Chromatographia34, 281 (1992).

    Google Scholar 

  14. C. F. Poole, T. O. Kollie, Anal. Chim. Acta282, 1 (1993).

    Google Scholar 

  15. S. K. Poole, C. F. Poole, J. Chromatogr. A697, 415 (1995).

    Google Scholar 

  16. S. K. Poole, C. F. Poole, Analyst120, 289 (1995).

    Google Scholar 

  17. M. H. Abraham, J. Phys. Org. Chem.6, 660 (1993).

    Google Scholar 

  18. M. H. Abraham, H. S. Chadha, A. J. Leo, J. Chromatogr. A685, 203 (1994).

    Google Scholar 

  19. S. K. Poole, C. F. Poole, Analyst (in press).

  20. M. L. Larrivee, C. F. Poole, Anal. Chem.66, 139 (1994).

    Google Scholar 

  21. P. C. Sadek, P. W. Carr, R. M. Doherty, M. J. Kamlet, R. W. Taft, M. H. Abraham, Anal. Chem.57, 2971 (1985).

    Google Scholar 

  22. D. E. Leahy, P. W. Carr, R. S. Pearlman, R. W. Taft, M. J. Kamlet, Chromatographia21, 473 (1986).

    Google Scholar 

  23. P. W. Carr, R. M. Doherty, M. J. Kamlet, R. W. Taft, W. Melander, Cs. Horváth, Anal. Chem.58, 2674 (1986).

    Google Scholar 

  24. M. J. Kamlet, M. H. Abraham, P. W. Carr, R. M. Doherty, R. W. Taft, J. Chem. Soc. Perkin Trans.2, 2087 (1988).

    Google Scholar 

  25. J. H. Park, P. W. Carr, M. H. Abraham, R. W. Taft, R. M. Doherty, M. J. Kamlet, Chromatographia25, 373 (1988).

    Google Scholar 

  26. J. H. Park, J. J. Chae, T. H. Nah, M. D. Jang, J. Chromatogr. A664, 149 (1994).

    Google Scholar 

  27. K. G. Miller, C. F. Poole, J. High Resolut. Chromatogr.17, 125 (1994).

    Google Scholar 

  28. M. L. Mayer, C. F. Poole, M. P. Henry, J. Chromatogr. A695, 267 (1995).

    Google Scholar 

  29. M. L. Mayer, S. K. Poole, C. F. Poole, J. Chromatogr. A697, 89 (1995).

    Google Scholar 

  30. D. S. Seibert, C. F. Poole, J. High Resolut. Chromatogr.18, 226 (1995).

    Google Scholar 

  31. C. F. Poole, S. K. Poole, “Chromatography Today”, Elsevier, Amsterdam, The Netherlands, 1991, p. 345.

    Google Scholar 

  32. M. H. Abraham, J. Andonian-Haftvan, G. S. Whiting, A. Leo, R. S. Taft, J. Chem. Soc. Perkin Trans.2, 1777 (1994).

    Google Scholar 

  33. M. H. Abraham, J. Phys. Org. Chem.6, 660 (1993).

    Google Scholar 

  34. M. H. Abraham, J. C. McGowan, Chromatographia23, 243 (1987).

    Google Scholar 

  35. R. S. S. Murthy, L. Crane, C. E. Bronniman, J. Chromatogr.542, 205 (1991).

    Google Scholar 

  36. C. F. Poole, S. K. Poole, “Chromatogrpahy Today”, Elsevier, Amsterdam, The Netherlands, 1991, p. 371.

    Google Scholar 

  37. K. S. Yun, C. Zhu, J. F. Parcher, Anal. Chem.67, 613 (1995).

    Google Scholar 

  38. Y. V. Kazakevic, H. M. McNair, J. Chromatogr. Sci.31, 317 (1993).

    Google Scholar 

  39. H. J. Mockel, U. Dreyer, H. Melzer, Fresenius' J. Anal. Chem.342, 673 (1992).

    Google Scholar 

  40. A. Alhedai, D. E. Martire, R. P. W. Scott, Analyst114, 869 (1989).

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Wayne State University, 48202, Detroit, MI, USA

    D. S. Seibert & C. F. Poole

  2. Zeneca/Smithkline Beecham Centre for Analytical Chemistry, Imperial College of Science, Technology, and Medicine, South Kensington, SW7 2AY, London, UK

    C. F. Poole

Authors
  1. D. S. Seibert
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  2. C. F. Poole
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Seibert, D.S., Poole, C.F. Influence of solvent effects on retention in reversed-phase liquid chromatography and solid-phase extraction using a cyanopropylsiloxane-bonded, silica-based sorbent. Chromatographia 41, 51–60 (1995). https://doi.org/10.1007/BF02274195

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  • DOI: https://doi.org/10.1007/BF02274195

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