Summary
The elution characteristics of polymeric solutes or inorganic salts in high-performance liquid chromatography were examined with a cellulose acetate column using water as the mobile phase. Polymeric (PVP, PEG, etc.) and ionic solutes eluted in a shorter time than deuterium oxide. The retention times for polymers decreased continuously as the molecular weight increased. The elution characteristic, of polymers is assumed to be affected mainly by size exclusion effect. The retention times for ionic solutes increased with the concentration of the injected solutions and asymptotically approached some constant values. The retention times were also affected by the valence and/or hydration of the ion. It was suggested tha the exclusion of ionic solutes was caused by electric repulsion and/or hydration of ion. It was recognized that the salts which were excluded more strongly were rejected more effectively by cellulose acetate membrane in reverse osmosis. HgCl2 which is not dissociated in aquous solution was observed to be adsorbed strongly on cellulose acetate.
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Kiso, Y., Kitao, T. Elution characteristics of polymeric solutes and inorganic salts in HPLC on a cellulose acetate column. Chromatographia 22, 341–344 (1986). https://doi.org/10.1007/BF02268787
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DOI: https://doi.org/10.1007/BF02268787