Summary
This paper describes the changes undergone by the layer dividing the vegetative from the generative cell. The abundant contents of the space inside the membrane, consisting of opaque bodies, vary considerably as the pollen grain matures, and decrease in volumepari passu with the formation of the dense bodies in the plasm of the vegetative cells. The dense bodies constitute a layer of droplets surrounding the enveloping membrane of the generative cell within the cytoplasm of the vegetative cell.
Connexions have been observed between the dense bodies and the partition layer which seem to indicate that the latter is apparently responsible for their organization, and is the area in which the synthesis of their contents takes place as well. The deposition of the dense bodies on the plasmalemma of the pollen grain points to their participation in the formation of one or other of the layers in which the grain is enveloped.
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References
Anderson, L. E., 1939: Cytoplasmic inclusions in the male gametes ofLilium. Amer. J. Bot.26, 761–766.
Dangeard, P., 1956: Le vacuome des grains de pollen et sa coloration vitale. Protoplasma46, 152–159.
Diers, L., 1963: Elektronenmikroskopische Beobachtungen an der vegetativen Zelle im auskeimenden Pollenkorn vonOenothera hookeri. Z. Naturforsch.B 18 A, 1092–1097.
—, 1963: Elektronenmikroskopische Beobachtungen und die generative Zelle vonOenothera hookeri. Z. Naturforsch.B 18 D, 562–566.
Frey-Wyssling, A., J. F. López-Sáez, andK. Mühlethaler, 1964: Formation and development of the cell plate. J. Ultrastruct. Res.10, 422–432.
Geitler, L., 1935: Beobachtungen über die erste Teilung im Pollenkorn der Angiospermen. Planta24, 361–386.
Giménez-Martín, G., M. C. Risueño, andJ. F. López-Sáez, 1967: A simple staining technique for electron microscopy with lead-uranyl acetate. Experientia23, 316.
Hofmeister, L., 1956: Über die Plasmagrenzschichten im Pollenkorn. Protoplasma46, 367–379.
Kostriukova, X. J., 1941: On the pellicular layer of the cytoplasm of the generative cell ofConvallaria majalis L. Comp. Rend. (Doklady) de l'Academie des Sciences de l'URSS.
López-Sáez, J. F., G. Giménez-Martín, andM. C. Risueño, 1966: Fine structure of the plasmodesm. Protoplasma61, 81–84.
—,M. C. Risueño, andG. Giménez-Martín, 1966: Inhibition of cytokinesis in plant cell. J. Ultrastruct. Res.14, 85–94.
Pinto-Lopes, J., 1949: On the differentiation of the nuclei in pollen grains of angiosperm. Port. Acta biol.2 A, 237–247.
Porter, K. R., andR. D. Machado, 1960: Studies on the endoplasmic reticulum. J. Biophys. Biochem. Cytol.7, 167–180.
Risueño, M. C., G. Giménez-Martín, andJ. F. López-Sáez, 1968: Experimental analysis of plant cytokinesis. Exp. Cell Res.49, 136–147.
Steffen, K., 1953: Zytologische Untersuchungen an Pollenkorn und Pollenschlauch. Flora140, 140–174.
Strasburger, E., 1908: Chromosomenzahlen, Plasmastructuren, Vererbungsträger und Reduktionsteilung. Jb. wiss. Bot.45, 479–568.
Suita, N., 1937 a: On the mature pollen grains in angiosperm. Bot. Mag.51, 524–529.
—, 1937 b: Studies on the ♂ gametophyte in angiosperms. II. Differentiation and behaviour of the vegetative and germinative elements in the pollen grains ofCrinum. Cytologia, Fujii jub.2, 920–933.
Vazart, B., 1958: Differenciation des cellules sexuelles et fecondation chez les phanerogames. Protoplasmatologia7, 3–158.
Whaley, W. G., andH. H. Mollenhauer, 1963: The Golgi apparatus and cell plate formation-a postulate. J. Cell Biol.17, 216–221.
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Giménez-Martín, G., Risueño, M.C. & López-Sáez, J.F. Generative cell envelope in pollen grains as a secretion system, a postulate. Protoplasma 67, 223–235 (1969). https://doi.org/10.1007/BF01248742
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DOI: https://doi.org/10.1007/BF01248742