Summary
RNA synthesis in giant cells containing polytene chromosomes in the embryo suspensor ofPhaseolus coccineus was analyzed by autoradiography after [3H]-uridine treatment. Embryos at the heart-shaped stage of development and at a cotyledonary stage were studied. Discontinuous labelling of the polytene chromosomes was always observed. The chromosomes were subdivided into segments (chromosome regions) which behaved as functional units, since discontinuous labelling was never seen within any of the regions. It was found that most chromosome regions were engaged in RNA synthesis to different degrees at the two embryo developmental stages. Regions showing identical labelling patterns tended to lie close together in the chromosome arms and to keep their functional activity coordinated at both stages of embryo development. The chromosome regions bearing 18 S+25 S ribosomal genes were never simultaneously active in RNA synthesis and different regions were preferentially transcribed at each stage of embryo development. However, at both stages, all the chromosome regions bearing 5 S ribosomal genes showed comparable labelling frequencies. The effect on transcription of gibberellic acid (GA3) treatments was also studied. At both embryo developmental stages, GA3 enhanced the rate of RNA synthesis in the polytene suspensor cells. The frequency with which certain chromosome regions were transcribed was also increased significantly (P⩽0.001) and this stimulatory effect was greater in embryos at the cotyledonary stage than in heart-shaped embryos. At the latter developmental stage, RNA synthesis was repressed by GA3 in a few chromosome regions. These results are discussed briefly in relation to previous findings using different methods of studying the organization of polytene chromosomes and the functional activity of the embryo suspensor ofPhaseolus coccineus.
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Alpi A (1989) Gibberellins in embryo development. Giorn Bot Ital 123: 311–320
—, Tognoni F, D'Amato F (1975) Growth regulator levels in embryo and suspensor ofPhaseolus coccineus at two stages of development. Planta 127: 153–162
Brady T (1973) Feulgen cytophotometric determination of the DNA content of the embryo proper and suspensor cells ofPhaseolus coccineus. Cell Differ 2: 67–75
Cionini PG (1987) The suspensor and its role in embryo development inPhaseolus (Papilionaceae): a review. Atti Soc Tosc Sci Nat Mem Ser B 94: 151–161
—, Bennici A, Alpi A, D'Amato F (1976) Suspensor, gibberellin and in vitro development ofPhaseolus coccineus embryos. Planta 131: 115–117
Cionini PG, Cavallini A, Corsi R, Folgi M (1982) Comparison of homologous polytene chromosomes inPhaseolus coccineus embryo suspensor cells: morphological, autoradiographic and cytochemical analyses. Chromosoma 86: 383–396
Clutter M, Brady T, Walbot V, Sussex I (1974) Macromolecular synthesis during plant embryogeny. Cellular rates of RNA synthesis in diploid and polytene cells in bean embryos. J Cell Biol 63: 1097–1102
Cremonini R, Cionini PG (1977) Extra DNA synthesis in embryo suspensor cells ofPhaseolus coccineus. Protoplasma 91: 303–313
Durante M, Cionini PG, Avanzi S, Cremonini R, D'Amato F (1977) Cytological localization of the genes for the four classes of ribosomal RNA (25 S, 18 S, 5.8 S and 5 S) in polytene chromosomes ofPhaseolus coccineus. Chromosoma 60: 269–282
—, Cremonini R, Tagliasacchi AM, Forino LMC, Cionini PG (1987) Characterization and chromosomal localization of fast renaturing and satellite DNA sequences inPhaseolus coccineus. Protoplasma 137: 100–108
Frediani M, Forino LMC, Tagliasacchi AM, Cionini PG, Durante M, Avanzi S (1986) Functional heterogeneity, during early embryogenesis, ofPhaseolus coccineus ribosomal cistrons in polytene chromosomes of embryo suspensor. Protoplasma 132: 51–57 Lima-De-Faria A, Pero R, Avanzi S, Durante M, Ståhle U.
D'Amato F, Granström H (1975) Relation between ribosomal RNA genes and the DNA satellites ofPhaseolus coccineus. Heredity 79: 5–20
Nagl W (1967) Riesenchromosomen vonPhaseolus coccineus L.: Baueigentümlichkeiten, Strukturmodifikationen, zusätzliche Nukleolen und Vergleich mit dem mitotischen Chromosomen. Österr Bot Z 114: 171–182
— (1974) ThePhaseolus suspensor and its polytene chromosomes. Z Pflanzenphysiol 73: 1–44
— (1979) Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. Plant Syst Evol [Suppl 2]: 247–260
Walbot V, Brady T, Clutter M, Sussex I (1972) Macromolecular synthesis during plant embryogeny: rates of RNA synthesis inPhaseolus coccineus embryos and suspensors. Dev Biol 29: 104–111
White P (1954) The cultivation of animal and plant cells. Roland Press, New York
Yeung EC, Sussex IM (1979) Embryogeny ofPhaseolus coccineus: the suspensor and the growth of the embryo-proper in vitro. Z Pflanzenphysiol 91: 423–433
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Forino, L.M.C., Tagliasacchi, A.M., Cavallini, A. et al. RNA synthesis in the embryo suspensor ofPhaseolus coccineus at two stages of embryogenesis, and the effect of supplied gibberellic acid. Protoplasma 167, 152–158 (1992). https://doi.org/10.1007/BF01403378
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DOI: https://doi.org/10.1007/BF01403378