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A polyclonal antibody (anticentrin) distinguishes between two types of fibrous flagellar roots in green algae

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Summary

The two main types of fibrous flagellar roots present in the flagellar apparatus of green algae (system I and system II fibers) are immunologically distinct as indicated by the localization of a Ca2+-modulated contractile protein (centrin) exclusively in one type (system II fibers) but not in the other type (system I fibers). A polyclonal antibody generated against the major protein of the striated flagellar roots (system II fibers) of the quadriflagellate green algaTetraselmis striata was used to localize centrin by immunofluorescence and pre- and postembedding immunogold electron microscopy in the flagellar apparatus ofSpermatozopsis similis, S. exsultans, Chlamydomonas reinhardtii, Dunaliella bioculata, Polytomella parva and gametes ofMonostroma grevillei andEnteromorpha sp. Whereas the antibody recognizes centrin in connecting fibers and system II fibers, no labeling occurs in system I fibers in all taxa investigated. This study presents the first evidence that system I fibers lack centrin and indicates that the two main types of fibrous flagellar roots in green algae are biochemically distinct.

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Authors and Affiliations

  1. Botanisches Institut, Westfälische Wilhelms-Universität, Münster

    M. Melkonian & D. Schulze

  2. Plant Cell Biology Centre, University of Melbourne, Melbourne

    G. I. McFadden

  3. Arbeitsgruppe Zellbiologie, Westfälische Wilhelms-Universität, Münster

    H. Robenek

Authors
  1. M. Melkonian
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  2. D. Schulze
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  3. G. I. McFadden
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  4. H. Robenek
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Melkonian, M., Schulze, D., McFadden, G.I. et al. A polyclonal antibody (anticentrin) distinguishes between two types of fibrous flagellar roots in green algae. Protoplasma 144, 56–61 (1988). https://doi.org/10.1007/BF01320280

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  • DOI: https://doi.org/10.1007/BF01320280

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