Skip to main content
Log in

Uromyces viciae-fabae onVicia faba: Scanning electron microscopy of frozen-hydrated material

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The ultrastructure of uredia, intercellular and intracellular hyphae ofUromyces viciae-fabae onVicia faba has been studied by scanning electron microscopy of frozen-hydrated material. Frozen-hydrated leaves will readily fracture and can be viewed on the cold stage of the microscope at liquid nitrogen temperatures for several hours without visible surface changes occurring. Both fungal and host-plant cells show a high degree of morphological preservation with little or no loss of cell contents and with minimal signs of shrinkage. Intracellular hyphae may be filamentous and branched or short and lobed. They are constricted at the point of entry into the host cell. Intercellular matrix material is well preserved around developing pedicels and urediospores and may act as a lubricant. The technique described is very quick compared with conventional preparatory procedures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beckett, A.,Porter, R.,Read, N. D., 1982: Low temperature scanning electron microscopy of fungal material. J. Microscopy (in press).

  • Bole, B., Parsons, E., 1973: Scanning electron microscopy of the internal cellular structure of plants. J. Microscopy98, 91–97.

    Google Scholar 

  • Boyde, A., Bailey, E., Jones, S. J., Tamarin, A., 1977: Dimensional changes during specimen preparation for scanning electron microscopy. Scanning Electron MicroscopyI, 507–518.

    Google Scholar 

  • —,Boyde, S., 1980: Further studies of specimen volume changes during processing for SEM: including some plant tissue. Scanning Electron MicroscopyII, 117–124.

    Google Scholar 

  • Echlin, P., Moreton, R. B., 1976: Low temperature techniques for scanning electron microscopy. Proc. 9th Ann. IITRI-SEM Symp.I, 753–762.

    Google Scholar 

  • —,Burgess, A., 1977: Cryofracturing and low temperature microscopy of plant material. Proc. 10th Ann. IITRI-SEM Symp.I, 491–500.

    Google Scholar 

  • —,Hayes, T. L., Pawley, J. B., 1979a: Freeze-fracture SEM of differentiating phloem parenchyma ofLemna minor L. (Duckweed). Beitr. elektronenmikroskop. Direktabb. Oberfl.12, 95–104.

    Google Scholar 

  • —,Pawley, J. B., Hayes, T. L., 1979 b: Freeze-fracture scanning electron microscopy ofLemna minor L. (Duckweed). Scanning Electron MicroscopyIII, 69–76.

    Google Scholar 

  • —,Lai, C. E., Hayes, T. L., Hook, G., 1980 a: Elemental analysis of frozen-hydrated differentiating phloem parenchyma in roots ofLemna minor L. Scanning Electron MicroscopyII, 383–394.

    Google Scholar 

  • —,Sauberman, A., 1980 b: Cryo fixation ofLemna minor roots for morphological and analytical studies. Cryo-LettersI, 289–298.

    Google Scholar 

  • Gold, R. E., Littlefield, L. J., 1979: Light and scanning electron microscopy of the telial, pycnial, and aecial stages ofMelampsora lini. Canad. J. Bot.57, 629–638.

    Google Scholar 

  • —,Statler, G. D., 1979: Ultrastructure of the pycnial and aecial stages ofPuccinia recondita. Canad. J. Bot.57, 74–86.

    Google Scholar 

  • Hassan, Z. M., Littlefield, L. J., 1979: Ontogeny of the uredium ofMelampsora lini. Canad. J. Bot.57, 639–649.

    Google Scholar 

  • Jones, D., 1980: Scanning electron microscopy of cryo-fixed sporodochia ofVolutella ciliata. Trans. Brit. mycol. Soc.75, 311–312.

    Google Scholar 

  • Littlefield, L. J., Heath, M. C., 1979: Ultrastructure of rust fungi; 277 p. New York-San Francisco-London: Academic Press.

    Google Scholar 

  • Michelmore, R. W., Ingram, D. S., 1981: Origin of gametangia in heterothallic isolates ofBremia lactucae. Trans. Brit. mycol. Soc.76, 425–432.

    Google Scholar 

  • Nei, T., Yotsumoto, H., Hasegawa, Y., Hasegawa, M., 1974: Freeze-etching in scanning electron microscopy. J. Electron Microscopy23, 137–138.

    Google Scholar 

  • O'Donnell, K. L., Hooper, G. R., Fields, W. G., 1976: Apothecium morphogenesis inPeziza quelepidotia: scanning electron and light microscopy. Canad. J. Bot.54, 2254–2267.

    Google Scholar 

  • —, 1977: Cryo fracturing as a technique for the study of fungal structures in the scanning electron microscope. Mycologia69, 309–320.

    Google Scholar 

  • Pawley, J. B., Norton, J. T., 1978: A chamber attached to the SEM for fracturing and coating frozen biological samples. J. Microscopy112, 169–182.

    Google Scholar 

  • Robards, A. W., 1974: Ultrastructural methods for looking at frozen cells. Sci. Prog. (Oxf.)61, 1–40.

    Google Scholar 

  • —,Crosby, P., 1979: A comprehensive freezing, fracturing and coating system for low temperature scanning electron microscopy. Scanning Electron MicroscopyII, 325–343.

    Google Scholar 

  • Tokunaga, J., Tokunaga, M., Yotsumoto, H., Hataba, Y., 1973: Cryo-scanning electron microscopy of conidiospore formation inAspergillus niger. Japan. J. Med. Mycol.14, 39–48.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beckett, A., Porter, R. Uromyces viciae-fabae onVicia faba: Scanning electron microscopy of frozen-hydrated material. Protoplasma 111, 28–37 (1982). https://doi.org/10.1007/BF01287644

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01287644

Keywords

Navigation