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Luminol chemiluminescence HPLC reaction detector for amino acids and other ligands

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Abstract

A detector for HPLC is based on suppression of chemiluminescence from the Cu(II)-luminol-peroxide reaction. Analytes which complex Cu(II) reduce the free Cu(II) concentration and thus the observed chemiluminescence intensity. The degree of chemiluminescence suppression is a measure of the analyte concentrations. Detection limits are in the range of 1 pmole-1 nmole for amino acids (both primary and secondary without derivatization), CN−, amines, catecholamines, catechol, and aminoglycoside antibiotics. The detection approach is demonstrated with an ion-exchange separation of amino acids, a reverse phase separation of catecholamines, and an ion-pair separation of the three components of gentamicin C in commercial gentamicin sulfate.

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Authors and Affiliations

  1. Department of Chemistry, University of Illinois, 1209 W. California Street, 61801, Urbana, IL, USA

    P. J. Koerner Jr. & Timothy A. Nieman

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  1. P. J. Koerner Jr.
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  2. Timothy A. Nieman
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Koerner, P.J., Nieman, T.A. Luminol chemiluminescence HPLC reaction detector for amino acids and other ligands. Mikrochim Acta 92, 79–90 (1987). https://doi.org/10.1007/BF01201720

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  • DOI: https://doi.org/10.1007/BF01201720

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