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Simultaneous determination of yohimbine and boldine by first-derivative synchronous spectrofluorimetry

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Abstract

A method for the simultaneous determination of yohimbine and boldine in mixtures by first-derivative synchronous spectrofluorimetry has been developed. The method is based on their native fluorescence in 0.1N sulphuric acid medium. The constant wavelength difference chosen to optimize the determination was Δλ=λem -λem=82 nm. Yohimbine was measured at λex//λem= 285/367 nm, and boldine at λex/λem=272/354 nm. The range of application is 10–500 μg/l for yohimbine and 1–50 μg/l for boldine. The method was applied to the determination of yohimbine and boldine in synthetic mixtures and pharmaceuticals, with errors generally ⩽2%. Relative standard deviations were about 2%.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Granada, E-18071, Granada, Spain

    José Luis Vilchez, Gonzalo Sánchez-Palencia, Ramiro Avidad, Luis Fermín Capitán-Vallvey & Alberto Navalón

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  1. José Luis Vilchez
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  2. Gonzalo Sánchez-Palencia
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  3. Ramiro Avidad
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  4. Luis Fermín Capitán-Vallvey
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  5. Alberto Navalón
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Dedicated to Professor Fermin Capitán on his 72th birthday

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Vilchez, J.L., Sánchez-Palencia, G., Avidad, R. et al. Simultaneous determination of yohimbine and boldine by first-derivative synchronous spectrofluorimetry. Mikrochim Acta 110, 61–69 (1993). https://doi.org/10.1007/BF01243986

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  • DOI: https://doi.org/10.1007/BF01243986

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