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A genetic linkage map of the rat derived from recombinant inbred strains

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Abstract

We have constructed a genetic linkage map in the rat by analyzing the strain distribution patterns of 500 genetic markers in a large set of recombinant inbred strains derived from the spontaneously hypertensive rat and the Brown-Norway rat (HXB and BXH recombinant inbred strains). 454 of the markers could be assigned to specific chromosomes, and the amount of genome covered by the mapped markers was estimated to be 1151 centimorgans. By including a variety of morphologic, biochemical, immunogenetic, and molecular markers, the current map integrates and extends existing linkage data and should facilitate rat gene mapping and genetic studies of hypertension and other complex phenotypes of interest in the HXB and BXH recombinant inbred strains.

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References

  • Belknap, J.K. (1992). Empirical estimates of Bonferroni corrections for use in chromosome mapping studies with the BXD recombinant inbred strains. Behav. Genet. 22, 677–684.

    Article  PubMed  CAS  Google Scholar 

  • Bender, K., Siebert, R.T., Wienker, T.F., Křen, V., Pravenec, M., Bissbort, S. (1994). Biochemical genetics of methylglyoxal dehydrogenases in the laboratory rat (Rattus norvegicus). Biochem. Genet. 32, 147–154.

    Article  PubMed  CAS  Google Scholar 

  • Butcher, G.W. (1987). A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenet. 14, 163–176.

    Article  PubMed  CAS  Google Scholar 

  • Cicila, G.T., Rapp, J.P., Wang, J.-M., St.Lezin, E., Ng, S.C., Kurtz, T.W. (1993). Linkage of 11b-hydroxylase mutations with altered steroid biosynthesis and blood pressure in the Dahl rat. Nature Genet. 3, 346–353.

    Article  PubMed  CAS  Google Scholar 

  • Crabbe, J.C., Belknap, J.K., Buck, K.J. (1994). Genetic animal models of alcohol and drug abuse. Science 264, 1715–1723.

    Article  PubMed  CAS  Google Scholar 

  • Cramer, D.V., Mowery, P.A., Adams, M. (1986). Biochemical markers in rats: linkage relationships of aconitase (Acon-1), aldehyde dehydrogenases (Ahd-2 and Ahd-c), alkaline phosphatase (Akp-1), and hydroxyacid oxidase (Hao-1). Biochem. Genet. 24, 217–227.

    CAS  Google Scholar 

  • Deng, A.Y., Rapp, J.P. (1992). Cosegregation of blood pressure with angiotensin converting enzyme and atrial natriuretic peptide receptor genes using Dahl salt-sensitive rats. Nature Genet. 1, 267–272.

    Article  PubMed  CAS  Google Scholar 

  • Deng, A.Y., Dene, H., Pravenec, M., Rapp, J.P. (1994). Genetic mapping of two new blood pressure quantitative trait loci in the rat by genotyping endothelin system genes. J. Clin. Invest. 93, 2701–2709.

    Article  PubMed  CAS  Google Scholar 

  • Dietrich, W.F., Miller, J.C., Steen, R.G., Merchant, M., Damron, D., Mahf, R., Gross, A., Joyce, D.C., Wessel, M., Dredge, R.D., Marquis, A., Stein, L.D., Goodman, N., Page, D.C., Lander, E.S. (1994). A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genet. 7(Suppl.), 220–245.

    Article  PubMed  CAS  Google Scholar 

  • Goldmuntz, E.A., Remmers, E.F., Zha, H., Cash, J.M., Mathern, P., Crofford, L.J., Wilder, R.L. (1993). Genetic map of 12 polymorphic loci on rat chromosome 1. Genomics 16, 761–764.

    Article  PubMed  CAS  Google Scholar 

  • Gora-Maslak, G., McClearn, G.E., Crabbe, J.C., Phillips, T.J., Belknap, J.K., Plomin, R. (1991). Use of recombinant inbred strains to identify quantitative trait loci in psychopharmacology. Psychopharmacology (Berlin) 104, 413–424.

    Article  CAS  Google Scholar 

  • Hamet, P., Kong, D., Pravenec, M., Kunes, J., Křen, V., Klír, P., Sun, Y.-L., Tremblay, J. (1992). RFLP of a HSP70 gene, localized in the RT1 complex, is associated with hypertension in SHR. Hypertension 19, 611–614.

    PubMed  CAS  Google Scholar 

  • Hedrich, H.J. (1992). Recombinant inbred strains. In Genetic Monitoring of Inbred Strains of Rats, H.J. Hedrich, ed. (Stuttgart: Gustav Fischer Verlag), pp. 487–494.

    Google Scholar 

  • Jacob, H.J., Brown, D.M., Bunker, R.K., Daly, M.J., Dzau, V.J., Goodman, A., Koike, G., Křen, V., Kurtz, T.W., Lernmark, A., Levan, G., Mao, Y.-p., Pettersson, A., Pravenec, M., Simon, J.S., Szpirer, C., Szpirer, J., Trolliet, M.R., Winer, E.S., Lander, E.S. (1995). A genetic linkage map of the laboratory rat, Rattus norvegicus. Nature Genet. 9, 63–69.

    Article  PubMed  CAS  Google Scholar 

  • Jeffreys, A.J., Wilson, V., Kelly, R., Taylor, B., Bulfield, G. (1987). Mouse DNA fingerprints: analysis of chromosome localization and germ-line stability of hypervariable loci in recombinant inbred strains. Nucleic Acids Res. 15, 2823–2833.

    Article  PubMed  CAS  Google Scholar 

  • Kendall, P.B. (1983). The genetics of esterase 12 and 13 polymorphisms in the Norway rat. Lab. Anim. 17, 221–224.

    Article  PubMed  CAS  Google Scholar 

  • Kershaw, E.E., Chua, S.C., Williams, J.A., Murphy, E.M., Leibel, R.L. (1995). Molecular mapping of SSRs for Pgml and C8b in the vicinity of the rat fatty locus. Genomics 27, 149–154.

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi, E., Tachibana, M., Ikadai, H., Imamichi, T., Kunieda T. (1992). Linkage among esterase-6 (Es-6), neural cell adhesion molecule (NCAM), and apolipoprotein C-III (apoc-3) genes on rat Chromosome 8. Mamm. Genome 3, 656–658.

    Article  PubMed  CAS  Google Scholar 

  • Křen, V. (1975). Genetics of the polydactyly-luxate syndrome in the Norway rat, Rattus norvegicus. Acta Univ. Carol. Med. (Praha), Monogr. 68, pp. 1–104.

    Google Scholar 

  • Křen, V., Pravenec, M., Bílá, V., Daniel, R., Hněvkovský, P., Kurtz, T.W. (1990). Genetic analysis of polydactyly-luxate syndrome. Transplant. Proc. 22, 2588–2589.

    PubMed  Google Scholar 

  • Křen, V., Pravenec, M., Bílá, V., Svobodová, D., St.Lezin, E., Kurtz, T.W. (1993). Linkage between the RT8 alloantigen and interleukin 6 loci on rat chromosome 4. Transplant. Proc. 25, 2777.

    PubMed  Google Scholar 

  • Kurtz, T.W., Simonet, L., Křen, V., Pravenec, M. (1991). Gene mapping in experimental hypertension. In Genetic Approaches to Coronary Heart Disease and Hypertension, K. Berg, V. Bulyzhenkov, Y. Christen, P. Corvol, eds. (Heidelberg: Springer-Verlag), pp. 38–59.

    Google Scholar 

  • Manly, K.F. (1993). A Macintosh program for storage and analysis of experimental mapping data. Mamm. Genome 4, 303–313.

    Article  PubMed  CAS  Google Scholar 

  • Mariat, D., Vergnaud, G. (1992). Detection of polymorphic loci in complex genomes with synthetic tandem repeats. Genomics 12, 454–458.

    Article  PubMed  CAS  Google Scholar 

  • Mariat, D., De Gouyon, B., Julier, C., Lathrop, M., Vergnaud, G. (1993). Genetic mapping through the use of synthetic tandem repeats in the mouse genome. Mamm. Genome 4, 135–140.

    Article  PubMed  CAS  Google Scholar 

  • Neumann, P.E. (1990). Two-locus linkage analysis using recombinant inbred strains and Bayes’ theorem. Genetics 126, 277–284.

    PubMed  CAS  Google Scholar 

  • Neumann, P.E. (1992). Inference in linkage analysis of multifactorial traits using recombinant inbred strains of mice. Behav. Genet. 22, 665–676.

    Article  PubMed  CAS  Google Scholar 

  • Neumann, P.E., Collins, R.L. (1991). Genetic dissection of susceptibility to audiogenic seizures in inbred mice. Proc. Natl. Acad. Sci. USA 88, 5408–5412.

    Article  PubMed  CAS  Google Scholar 

  • Plomin, R., McClearn, G.E., Gora-Maslak, G., Neiderhiser, J.M. (1991). Use of recombinant inbred strains to detect quantitative trait loci associated with behavior. Behav. Genet. 21, 99–116.

    Article  PubMed  CAS  Google Scholar 

  • Pravenec, M., Klir, P., Křen, V., Zicha, J., Kunes, J. (1989). An analysis of spontaneous hypertension in spontaneously hypertensive rats by means of new recombinant inbred strains. J. Hypertens. 7, 217–222.

    Article  PubMed  CAS  Google Scholar 

  • Pravenec, M., Křen, V., Kabra, P., Bílá, V., Kršiaková, M., Simonet, L., Kurtz, T.W. (1990). HXB and BXH sets of recombinant inbred strains: strain distribution patterns of some markers. Transplant. Proc. 22, 2557–2558.

    PubMed  CAS  Google Scholar 

  • Pravenec, M., Křen, V., Kuneš, J., Scicli, A.G., Carretero, O.A., Simonet, L., Kurtz, T.W. (1991). Cosegregation of blood pressure with an RFLP in the kallikrein gene family. Hypertension 17, 242–246.

    PubMed  CAS  Google Scholar 

  • Pravenec, M., Simonet, L., Křen, V., St.Lezin, E., Levan, G., Szpirer, J., Szpirer, C., Kurtz, T.W. (1992). Assignment of rat linkage group V to chromosome 19 by single strand conformation polymorphism analysis of somatic cell hybrids. Genomics 12, 350–356.

    Article  PubMed  CAS  Google Scholar 

  • Pravenec, M., St.Lezin, E., Křen, V., Wang, J.-M., Kurtz, T.W. (1994). Linkage mapping of alkaline phosphatase 1, inhibin α subunit, and ³ crystallin 1 on rat chromosome 9 and Na+, K+-ATPase α2 subunit, renin, and leukocyte common antigen on rat chromosome 13. Genomics 19, 190–191.

    Article  PubMed  CAS  Google Scholar 

  • Pravenec, M., Gauguier, D., Schott, J.J., Buard, J., Křen, V., Bílá, V., Szpirer, C., Szpirer, J., Wang, J.-M., Huang, H., St.Lezin, E., Spence, M.A., Flodman, P., Printz, M., Lathrop, M., Vergnaud, G., Kurtz, T.W. (1995). Mapping of quantitative trait loci for blood pressure and cardiac mass in the rat by genome scanning of recombinant inbred strains, J. Clin. Invest. 96, 1973–1978.

    Article  PubMed  CAS  Google Scholar 

  • Remmers, E.F., Goldmuntz, E.A., Zha, H., Mathern, P., Du, Y., Crofford, L.J., Wilder, R.L. (1993). Linkage map of nine loci defined by polymorphic DNA markers assigned to rat chromosome 13. Genomics 18, 277–282.

    Article  PubMed  CAS  Google Scholar 

  • Serikawa, T., Kuramoto, T., Hilbert, P., Mori, M., Yamada, J., Dubay, C.J., Lindpainter, K., Ganten, D., Guenét, J.-L., Lathrop, M., Beckmann, J.J. (1992). Rat gene mapping using PCR-analyzed microsatellites. Genetics 131, 703–723.

    Google Scholar 

  • Silver, J. (1985). Confidence limits for estimates of gene linkage based on analysis of recombinant inbred strains. J. Hered. 76, 436–40.

    PubMed  CAS  Google Scholar 

  • Szpirer, C., Szpirer, J., Riviere, G., Levan, G., Orlowski, J. (1994a). Chromosomal assignment of four genes encoding Na/H exchanger isoforms in human and rat. Mamm. Genome 5, 153–158.

    CAS  Google Scholar 

  • Szpirer, C., Tissir, F., Riviere, M., Levan, G., Szpirer, J. (1994b). Assignment of rat Jun family genes to chromosome 19 (Junb), chromosome 5q31–33 (Jun), and chromosome 16 (Jund). Mamm. Genome 5, 361–364.

    Article  PubMed  CAS  Google Scholar 

  • Taylor, B.A. (1978). Recombinant inbred strains: Use in gene mapping. In: Origins of Inbred Mice, H.C. Morse III, ed. (New York: Academic Press), pp. 423–38.

    Google Scholar 

  • Taylor, B.A., Reifsnyder, P.C. (1993). Typing recombinant inbred mouse strains for microsatellite markers. Mamm. Genome 4, 239–242.

    Article  PubMed  CAS  Google Scholar 

  • van Zutphen, L.F., den Bieman, M.G. (1984). Rat esterases: an update of linkage group V. J. Hered. 75, 313.

    Google Scholar 

  • Vergnaud, G. (1989). Polymers of random short oligonucleotides detect polymorphic loci in human genome. Nucleic Acids Res. 17, 7623–7630.

    Article  PubMed  CAS  Google Scholar 

  • Vergnaud, G., Mariat, D., Apiou, F., Aurias, A., Lathrop, M., Lauthier, V. (1991a). The use of synthetic tandem repeats to isolate new VNTR loci: cloning of a human hypermutable sequence. Genomics 11, 135–144.

    Article  PubMed  CAS  Google Scholar 

  • Vergnaud, G., Mariat, D., Zoroastro, M., Lauthier, V. (1991b). Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides. Electrophoresis 12, 134–140.

    Article  PubMed  CAS  Google Scholar 

  • Vergnaud, G., Gauguier, D., Schott, J.J., Lepetit, D., Lauthier, V., Mariat, D., Buard, J. (1993). Detection, cloning, and distribution of minisatellites in some mammalian genomes. EXS 67, 47–57.

    PubMed  CAS  Google Scholar 

  • Yamada, J., Kuramoto, T., Serikawa, T. (1994). A rat genetic linkage map and comparative maps for mouse or human homologous rat genes. Mamm. Genome 5, 63–83.

    Article  PubMed  CAS  Google Scholar 

  • Zha, H., Wilder, R.L., Goldmuntz, E.A., Cash, J.M., Crofford, L.J., Mathern, P., Remmers, E.F. (1993). Linkage map of 10 polymorphic markers on rat chromosome 2. Cytogenet. Cell Genet. 63, 117–122.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic

    M. Pravenec

  2. Laboratoire de Genetique Moleculaire, Centre d’Etudes du Bouchet, Vert le Petit, France

    D. Gauguier, J. -J. Schott, J. Buard & G. Vergnaud

  3. Wellcome Trust Centre for Human Genetics, Oxford, UK

    D. Gauguier & G. M. Lathrop

  4. Laboratoire de Genetique des Especes, Institut de Biologie, Nantes, France

    J. -J. Schott, J. Buard & G. Vergnaud

  5. Department of Biology, 1st Medical Faculty, Charles University, Prague, Czech Republic

    V. Křen & V. Bílá

  6. Universite Libre de Bruxelles, Rhode-St-Genese, Department of Molecular Biology, Belgium

    C. Szpirer & J. Szpirer

  7. Department of Laboratory Medicine, University of California, San Francisco, California, USA

    J. -M. Wang, H. Huang, E. St.Lezin & T. W. Kurtz

  8. Department of Pediatrics, University of California, Irvine, California, USA

    M. A. Spence & P. Flodman

  9. Department of Pharmacology, University of California, San Diego, California, USA

    M. Printz

  10. INSERM U358, Hopital St. Louis, Paris, France

    G. M. Lathrop

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  1. M. Pravenec
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  2. D. Gauguier
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  3. J. -J. Schott
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  4. J. Buard
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  5. V. Křen
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  6. V. Bílá
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  7. C. Szpirer
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  8. J. Szpirer
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  9. J. -M. Wang
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  10. H. Huang
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  11. E. St.Lezin
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  12. M. A. Spence
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  13. P. Flodman
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  14. M. Printz
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  15. G. M. Lathrop
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  16. G. Vergnaud
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  17. T. W. Kurtz
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Pravenec, M., Gauguier, D., Schott, J.J. et al. A genetic linkage map of the rat derived from recombinant inbred strains. Mammalian Genome 7, 117–127 (1996). https://doi.org/10.1007/s003359900031

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  • DOI: https://doi.org/10.1007/s003359900031

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