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Ploidy reduction usingp-Fluorophenylalanine of fusion products ofSaccharomyces cerevisiae

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Abstract

Fusion of yeast protoplasts was induced in the presence of polyethylene glycol and Ca++ ions. Two auxotrophic complementingSaccharomyces cerevisiae mutant strains were used in fusion experiments. One diploid and several polyploid fusion products were selected by complementation in minimal medium. The assessment of ploidy has been based on the DNA content of the parental cells and fusion products, assayed with the diphenylamine method. Treating the fusion product cells withp-Fluorophenylalanine (p-FPA), parentalhis andleu markers could not be recovered. Instead, a strong reduction of polyploid fusion product cell DNA content was evident. The analysis of meiotic products after hybridizing one fusion product with a prototrophicSaccharomyces cerevisiae standard strain led to the recovery of thehis parental marker. Preliminary evidence thatp-Fluorophenylalanine could be used as a diploidizing agent towards polyploid strains ofSaccharomyces cerevisiae is reported.

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Literature Cited

  1. Ahkong, Q. F., Howell, J. I., Lucy, J. A. 1975. Fusion of hen erythrocytes with yeast protoplasts induced by polyethylene glycol. Nature225:66–67.

    Google Scholar 

  2. Arima, K., Takano, I. 1979. Multiple fusion of protoplasts inSaccharomyces cerevisiae yeasts. Molecular and General Genetics173:271–277.

    PubMed  Google Scholar 

  3. Anné, J., Peberdy, J. F. 1976. Induced fusion of fungal protoplasts following treatment with polyethylene glycol. Journal of General Microbiology92:413–417.

    PubMed  Google Scholar 

  4. Espósito, M. S., Espósito, R. E., Arnaud, M., Halvorson, H. O. 1969. Acetate utilization and macromolecular synthesis during sporulation of yeasts. Journal of Bacteriology100(1):180–186.

    PubMed  Google Scholar 

  5. Flores da Cunha, M. 1970. Mitotic mapping ofSchizosaccharomyces pombe. Genetical Research Cambridge16:127–144.

    Google Scholar 

  6. Fournier, P., Provost, A., Bourguignon, C., Heslot, H. 1977. Recombination after protoplast fusion in the yeastCandida tropicalis. Archives of Microbiology115:143–149.

    PubMed  Google Scholar 

  7. Fowell, R. R. 1951. Journal of the Institute of Brewing57:180–195.

    Google Scholar 

  8. Fowell, R. R. 1969. Life cycles of yeasts, p. 461. In: Rose, A., Harrison, J. S. (eds.), The yeasts, vol. 1. London, New York: Academic Press.

    Google Scholar 

  9. Fowell, R. R. 1969. Sporulation and hybridization of yeasts, p. 368. In: Rose, A., Harrison, J. S. (eds.), The yeasts, vol. 1. London, New York: Academic Press.

    Google Scholar 

  10. Gunge, N., Nakatomi, Y. 1962. Genetic mechanisms of rare matings of the yeastSaccharomyces cerevisiae heterozygous for mating type. Genetics70:41–58.

    Google Scholar 

  11. Gunge, N., Tamaru, A. 1978. Genetic analysis of products of protoplast fusion inSaccharomyces cerevisiae. The Japanese Journal of Genetics53(1):41–49.

    Google Scholar 

  12. Gutz, H. 1966. Induction of mitotic segregation withp-FPA inSchizosaccharomyces pombe. Journal of Bacteriology92(5):1567.

    PubMed  Google Scholar 

  13. Johnston, J. R., Mortimer, R. K. 1959. Use of snail digestive juice in isolation of yeast spore tetrads. Journal of Bacteriology78:292.

    PubMed  Google Scholar 

  14. Lindegren, C. C., Lindegren, G. 1943. Proceedings of the National Academy of Sciences U.S.A.29:306–308.

    Google Scholar 

  15. McCully, K., Forbes, E. 1965. The use ofp-Fluorophenylalanine with “master strains” ofAspergillus nidulans for assigning genes to linkage groups. Genetical Research6:352–359.

    PubMed  Google Scholar 

  16. Parry, J., Zimmermann, F. 1976. The detection of monosonic colonies produced by mitotic chromosome nondisjunction in the yeastSaccharomyces cerevisiae.

  17. Peberdy, J., Heyssen, H., Anné, J. 1977. Interspecific hybridization betweenPenicillium chrisogenum andPenicillium cyaneo-fulvum following protoplast fusion. Molecular and General Genetics157:281–284.

    Google Scholar 

  18. Peberdy, J. 1979. Fungal protoplasts: isolation, reversion and fusion. Annual Review on Microbiology33:21–39.

    Google Scholar 

  19. Peberdy, J. 1980. Protoplast fusion—a tool for genetic manipulation and breeding in industrial microorganisms. Enzyme and Microbial Technology2:23–29.

    Google Scholar 

  20. Provost, A., Bourguignon, C., Fournier, P., Ribet, A. M., Heslot, H. 1978. Intergeneric hybridization in yeasts through protoplast fusion. FEMS Microbiology Letters3:309–312.

    Google Scholar 

  21. Sipiczki, M., Ferenczy, L. 1977. Protoplast fusion ofSchizosaccharomyces pombe auxotrophic mutants of identical mating type. Molecular and General Genetics151:77–81.

    PubMed  Google Scholar 

  22. Sipiczki, M. 1979. Interspecific protoplast fusion in fission yeasts. Current Microbiology3:37–40.

    Google Scholar 

  23. Strömnaes, Ö. 1968. Genetic changes inSaccharomyces cerevisiae grown in media containing DL-p-Fluorophenylalanine. Hereditas59:197–220.

    Google Scholar 

  24. van Broock M., Sierra, M., de Figueroa, L. 1981. Intergeneric fusion of yeast protoplasts. Proceedings of the Fifth International Symposium of yeasts, pp. 171–176 In: Stewart, G. G., Russell, I. (eds.), Current developments in yeast research. Canada: Pergamon Press.

    Google Scholar 

  25. van Solingen, P., van der Plaat, J. 1977. Fusion of yeast spheroplasts. Journal of Bacteriology130:946–947.

    PubMed  Google Scholar 

  26. Wickerham, L. J., Burton, K. A. 1954. Journal of Bacteriology67:303–308.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. PROIMI-Planta Piloto de Procesos Industriales Microbiológicos, Avda. Belgrano y Pasaje Caseros, 4000, Tucumán, República Argentina

    María R. de van Broock, Mirta Sierra & Lucía C. de Figueroa

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  1. María R. de van Broock
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  2. Mirta Sierra
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  3. Lucía C. de Figueroa
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de van Broock, M.R., Sierra, M. & de Figueroa, L.C. Ploidy reduction usingp-Fluorophenylalanine of fusion products ofSaccharomyces cerevisiae . Current Microbiology 8, 13–16 (1983). https://doi.org/10.1007/BF01567307

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