Abstract
Legionella and amebae populations in 16 cooling towers were challenged with three commercially available biocide formulations. The active agents were: a chlorinated phenolic thioether (CPTE), bromo-nitro-propane-diol (BNPD), and bromo-chloro-dimethylhydantoin (BCD, in briquette form). The towers were dosed with these biocides for approximately 4 weeks. BCD was effective against Legionella in each of nine challenge experiments, and CPTE in eight of nine challenges. BNPD was effective in only five of 11 challenges. None of the biocides had any significant effect in reducing planktonic amebae concentrations during the challenges.
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Literature Cited
Australian Standard AS3666—1989. Air-handling and water systems of buildings—microbial control. Sydney: Standards Australia
Bentham RH (1993) Environmental factors affecting the colonization of cooling towers by Legionella spp. in South Australia. Int Biodeterior Biodegrad 31:55–65
Bentham RH, Broadbent CR, Marwood LN (1993a) The influence of the sessile population in the Legionella colonization of cooling towers. In: Barbaree JM, Breiman RF, Dufour AP (eds) Legionella—current status and emerging perspectives. Washington: American Society for Microbiology, CIP 92-48890, pp 267–269
Bentham RH, Broadbent CR, Marwood LN, Marsh J, McDonald PJ, Lee PC (1993b) Ecology and control of Legionella in cooling towers-report of a field study in Adelaide. Report of Australian construction services field study. Adelaide: Federal Department of Administrative Services, South Australia
Broadbent CR, Marwood LN, Bentham RH (1991) Legionella in cooling towers: report of a field study in South Australia. In: Parsons RA (ed) Proceedings of the ASHRAE Far East Conference on Environmental Quality. Atlanta: American Society of Heating, Refrigeration and Air-conditioning Engineers, pp 55–59
Dennis PJL (1988) Isolation of Legionellae from environmental specimens. In: Harrison TG, Taylor AG (eds) A laboratory manual for Legionella. New York: John Wiley, pp 31–43
Department of Health (UK) (1988) Report of the expert advisory committee on biocides, Wright AE (chairman). London: Her Majesty's Stationary Office
Edelstein PH (1985) Legionella. In: Lennette EH, Balows A, Hausler WJ Jr, Shadomy HJ (eds) Manual of clinical microbiology, 4th ed. Washington D.C.: American Soc. Microbiol., pp 373–379
Elsmore R (1986) Biocidal control of legionellae. Isr J Med Sci 22:647–654
England JC, Fraser DW, Mallison GW, Mackel DC, Skaliy P, Gorman G (1982) Failure of L. pneumophila sensitivities to predict culture results from disinfectant-treated air-conditioning cooling towers. Appl Environ Microbiol 43:240–244
Fields BS, Sanden GN, Barbaree JM, Morrill WE, Wadowsky RM, White EH, Feeley JC (1989) Intracellular multiplication of Legionella pneumophila in amoebae isolated from hospital hot water tanks. Curr Microbiol 18:131–137
Green PN, Pirrie RS (1993) A laboratory apparatus for the generation and biocide efficacy testing of Legionella biofilms. J Appl Bacteriol 74:388–393
Grow KM, Wood DH, Coggin JH, Leinbach ED (1984) Environmental factors influencing growth of L. pneumophila in operating biocide-treated cooling towers In: Thornsberry C, Balows A, Feeley JC, Jakubowski W (eds) Legionella: Proceedings of the Second International Legionella Symposium. Washington D.C.: American Society of Microbiology, pp 316–318
Henke M, Seidel KM (1986) Association between Legionella pneumophila and amoebae in water. Isr J Med Sci 22:690–695
Kurtz JB, Davis V (1988) Efficacy of bromine biocide in cooling water systems. Letter. Lancet i:304
Kurtz JB, Bartlett CLR, Newton UA, White RA, Jones NL (1982) L. pneumophila in cooling water systems. J Hyg Camb 88:369–381
National Health and Medical Research Council (Australia) (1989) Australian guidelines for the control of Legionella and Legionnaires' disease. Canberra: Australian Government Publishing Services
Page FC (1988) A new key to freshwater and soil Gymnamoebae. Cumbria, UK: Freshwater Biological Association
Rowbotham TJ (1980) Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol 33:1179–1183
Skaliy P, Thompson TA, Gorman GW, Morris GK, McEachern HV, Mackel DC (1980) Laboratory studies of disinfectants against Legionella pneumophila. Appl Environ Microbiol 40:697–700
Soracco RJ, Gill HK, Fliermans CB, Pope DH (1988) Susceptibilities of algae and Legionella pneumophila to cooling tower biocides. Appl Environ Microbiol 45:1254–1260
Srikanth S, Berk SG (1993) Stimulatory effect of cooling tower biocides on amoebae. Appl Environ Microbiol 59:3245–3249
Swango LJ, Wilt GR, Killen AD, Williams DE, Worley SD (1987) Inactivation of L. pneumophila by hypochlorite and an organic chloramine. Appl Environ Microbiol 53:2983–2986
Yamamoto H, Ezaki T, Ikedo M, Yabuuchi E (1991) Effects of biocidal treatments to inhibit the growth of legionellae and other microorganisms in cooling towers. Microbiol Immunol 35:795–802
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Bentham, R.H., Broadbent, C.R. Field trial of biocides for control of Legionella in cooling towers. Current Microbiology 30, 167–172 (1995). https://doi.org/10.1007/BF00296203
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DOI: https://doi.org/10.1007/BF00296203