Skip to main content
SpringerLink
Log in

Pulsed-field gel electrophoresis study of the genome ofCaldocellum saccharolyticum

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The genome of the anaerobic thermophilic eubacteriumCaldocellum saccharolyticum was analyzed by pulsed field gel electrophoresis. The restriction endonucleasesNheI andSstII cleaved the genome into 23 and 21 fragments, respectively, and the sums of the fragments gave a genome size of approximately 2.78 Mbp. Hybridization of probes for several cellulolytic and hemicellulolytic genes, as well as a 16S ribosomal RNA gene, gave complex signals on PFGE-separated restriction fragments. Mapping of the genome by use of linking clone hybridizations had been initiated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Albertson G (1992) PhD. thesis. University of Auckland

  2. Allardet-Servent A, Carles-Nurit M-J, Bourg G, Michaux S, Ramuz M (1991) Physical map of theBrucella melitensis 16M chromosome. J Bacteriol 173:2219–2224

    Google Scholar 

  3. Amjad M, Castro JM, Sandoval H, Wu J-J, Yang M, Henner DJ, Piggot PJ (1990) An SfiI restriction map of theBacillus subtilis 168 genome. Gene 101:15–21

    Google Scholar 

  4. Barany F (1988) Procedures for linker insertion mutagenesis and use of new kanamycin resistance cassettes. DNA and Prot. Eng. Tech. 1:29–34

    Google Scholar 

  5. Bergquist PL, Morgan WH (1991) The molecular genetics and biotechnological applications of enzymes from extremely thermophilic eubacteria. In: Herbert RH, Sharp RT (eds) Molecular biology and biotechnology of extremophiles. Edinburgh: Blackie and Sons, pp 44–75

    Google Scholar 

  6. Borges KM, Bergquist PL (1992) A rapid method for preparation of bacterial chromosomal DNA in agarose plugs usingThermus Rt41A proteinase. Bio Techniques 12:222–223

    Google Scholar 

  7. Borges KM, Bergquist PL (1993) Physical map of the genome of the extremely thermophilic bacteriumThermus thermophilus HD8. J Bacterial 175:103–110

    Google Scholar 

  8. Conrad B, Cole ST (1989) Genome organization of the anaerobic pathogenClostridium perfringens. Proc Natl Acad Sci USA 86:6676–6680

    Google Scholar 

  9. Donnison AM, Brocklesbury CM, Morgan WH (1986) A new species of non-sporulating thermophilic cellulolytic bacterium. Proceedings, International Congress of Microbiology, Manchester. Abstract P14/18

  10. Fritz RB, Musich PR (1990) Unexpected loss of genomic DNA from agarose gel plugs. Bio Techniques 9:542–550

    Google Scholar 

  11. Gibbs MD, Saul DJ, Lüthi E, Bergquist PL (1992) The β-mannanase from “Caldocellum saccharolyticum” is part of a multidomain enzyme. Appl Environ Microbiol 58:3864–3867

    Google Scholar 

  12. Hanahan D (1985) Techniques for transformation ofE. coli. In: Glover DM (ed), DNA cloning, vol. 1, A practical approach. Oxford: IRL Press, pp 109–135

    Google Scholar 

  13. Le Bourgeois P, Mata M, Ritzenthaler P (1989) Genome comparison ofLactococcus strains by pulsed-field gel electrophoresis. FEMS Microbiol Lett 59:65–70

    Google Scholar 

  14. Love DR, Fisher R, Bergquist PL (1988) Sequence structure and expression of a cloned β-glucosidase gene from an extreme thermophile. Mol Gen Genet 213:84–92

    Google Scholar 

  15. Lüthi E, Bhana Jasmat N, Bergquist PL (1990a) A xylanase from the extremely thermophilic bacterium “Caldocellum saccharolyticum”: Overexpression of the gene product inEscherichia coli and characterization of the gene product. Appl Environ Microbiol 56:2677–2683

    Google Scholar 

  16. Lüthi E, Bhana Jasmat N, Bergquist PL (1990b) Overproduction of an acetylxylan esterase from the extreme thermophile “Caldocellum saccharolyticum” inEscherichia coli. Appl Microbiol Biotechnol 34:214–219

    Google Scholar 

  17. Lüthi E, Bhana Jasmat N, Grayling RA, Love DR, Bergquist PL (1991) Cloning, sequence analysis, and expression inEscherichia coli of a gene coding for β-mannanase from the extremely thermophilic bacterium “Caldocellum saccharolyticum”. Appl Environ Microbiol 57:694–700

    Google Scholar 

  18. Noll KM (1989) Chromosome map of the thermophilic archaebacteriumThermococcus celer. J Bacteriol 171:6720–6725

    Google Scholar 

  19. Pattee P (1990) Genetic and physical mapping of the chromosome ofStaphylococcus aureus. In: Drlica K, Riley M (eds) The bacterial chromosome. Washington, DC: American Society for Microbiology, pp 163–169

    Google Scholar 

  20. Reeves R (University of Auckland). Personal communication

  21. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory Press

    Google Scholar 

  22. Saul DJ, Williams LC, Grayling RA, Chamley LW, Love DR, Bergquist PL (1990)CelB, a gene coding for a bifunctional cellulase from the extreme thermophile “Caldocellum saccharolyticum.” Appl Environ Microbiol 56:3117–3124

    Google Scholar 

  23. Saul DJ, Rodrigo A, Reeves R, Bergquist PL (1992) Unpublished data

  24. Smith CL, Condemine G (1990) New approaches for physical mapping of small genomes. J. Bacteriol 172:1167–1172

    Google Scholar 

  25. Smith CL, Klco SR, Cantor CR (1988) Pulsed-field electrophoresis and the technology of large DNA molecules. In: Davies K (ed) Genome analysis. Oxford: IRL Press Ltd, pp 41–72

    Google Scholar 

  26. Tanskanen EI, Tulloch DL, Hillier AJ, Davidson BE (1990) Pulsed-field gel electrophoresis ofSmaI digests of Lactococcal genomic DNA, a novel method of strain identification. Appl Environ Microbiol 56:3105–3111

    Google Scholar 

  27. Teo VT (1992) MSc. thesis, University of Auckland, New Zealand

  28. Yamagishi A, Oshima T (1990) Circular chromosomal DNA in the sulfur-dependent archaebacteriumSulfolobus acidocaldarius. Nucleic Acids Res 18:1133–1135

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand

    Kim M. Borges &  Peter L. Bergquist

Authors
  1. Kim M. Borges
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter L. Bergquist
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borges, K.M., Bergquist, P.L. Pulsed-field gel electrophoresis study of the genome ofCaldocellum saccharolyticum . Current Microbiology 27, 15–19 (1993). https://doi.org/10.1007/BF01576828

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01576828

Keywords

Search

Navigation