Abstract
This study evaluates the toxicity of lead in the presence or absence of cadmium on the maintenance of a monolayer of cultured bovine aortic endothelial cells. It was histologically shown that lead at 50 μM or less induced a slight formation of de-endothelialized areas on the monolayer after a 24-h incubation. Although lead accumulated in endothelial cells in a dose-dependent manner, the cytotoxicity (evaluated by the [3H]adenine release assay) was not detected. However, cadmium (1.0 or 2.0 μM)-induced de-endothelialized areas on the monolayer was considerably larger in the presence of lead at 10 μM; the cytotoxicity of cadmium was significantly enhanced by lead. It was concluded that vascular endothelial cell monolayer is severely destroyed by a greater cytotoxicity of combination of cadmium with lead.
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Kaji, T., Suzuki, M., Yamamoto, C. et al. Severe damage of cultured vascular endothelial cell monolayer after simultaneous exposure to cadmium and lead. Arch. Environ. Contam. Toxicol. 28, 168–172 (1995). https://doi.org/10.1007/BF00217612
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DOI: https://doi.org/10.1007/BF00217612