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Glucose-dependent respiration in suspensions of rabbit cortical tubules

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Summary

The effects of glucose on cellular respiration were examined in suspensions of rabbit cortical tubules. When glucose was removed from the bathing fluid, oxygen consumption (QO2) decreased from 18.6±0.8 to 15.7±0.5 nmol O2/mg protein·min (P<0.01). The transported but nonmetabolized analogue of glucose, α-methyl-d-glucoside (αMG), was found to support QO2 to the same extent as glucose. These observations were also evident in the presence of butyrate, a readily oxidized substrate of the renal cortex. Additional studies with nystatin and ouabain indicated that glucose-related changes in QO2 were the result of changes in Na, K-ATPase associated respiration. The effect of glucose was localized to the luminal membrane since phlorizin (10−5 m), a specific inhibitor of liminalk glucose-sodium cotransport, also significantly reduced QO2 by 10±1%. Phlorizin inhibition of QO2 was also evident in the presence of αMG but was abolished when glucose was removed from the bathing medium. Finally, measurement of NADH fluorescence showed that addition of glucose (5mm) to a tubule suspension causes an oxidation of NAD. These data are all consistent with glucose acting to increase respiration by stimulating sodium entry at the luminal membrane (via glucose-sodium cotransport) followed by increased sodium pump activity and its associated increase in mitochondrial respiration.

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References

  • Arthus, M.-F., Bergeron, M., Scriver, C.R. 1982. Topology of membrane exposure in the renal cortex slice: Studies of glutathione and maltose changes.Biochim. Biophys. Acta 692:371–376

    PubMed  Google Scholar 

  • Balaban, R.S., Soltoff, S.P., Storey, J.M., Mandel, L.J. 1980. Improved renal cortical tubule suspension: Spectrophotometric study of O2 delivery.Am. J. Physiol. 238:F50-F59

    PubMed  Google Scholar 

  • Barfuss, D.W., Schaffer, J.A. 1981. Differences in active and passive glucose transport along the proximal tubule.Am. J. Physiol. 290:F322-F332

    Google Scholar 

  • Brazy, P.C., Dennis, V.W. 1978. Characteristics of glucosephlorizin interactions in isolated proximal tubules.Am. J. Physiol. 234:F279-F286

    PubMed  Google Scholar 

  • Burg, M., Patlak, C., Green, N., Villey, D. 1976. Organic solutes in fluid reabsorption by renal proximal convoluted tubules.Am. J. Physiol. 231:627–637

    PubMed  Google Scholar 

  • Chesney, R., Sacktor, B., Kleinzeller, A. 1974. The binding of phlorizin to the isolated luminal membrane of the renal proximal tubule.Biochim. Biophys. Acta 332:263–277

    Google Scholar 

  • Cohen, J.J., Kamm, D.E. 1976. Renal metabolism: Relation to renal function.In: The Kidney, B.M. Brenner and F.C. Rector, editors. pp. 126–214. Saunders, Philadelphia

    Google Scholar 

  • Frasch, W., Frohnert, P.P., Boxe, F., Baumann, K., Kinne, R. 1970. Competitive inhibition of phlorizin binding byd-glucose and the influence of sodium: A study on isolated brush border membrane of rat kidney.Pfluegers Arch. 320:265–284

    Article  Google Scholar 

  • Frömter, E. 1982. Electrophysiological analysis of rat renal sugar and amino acid transport: I. Basic phenomena.Pfluegers Arch. 393:179–189

    Article  Google Scholar 

  • Gornall, A.G., Bardawill, C.J., David, M.M. 1949. Determination of serum proteins by means of the biuret reaction.J. Biol. Chem. 177:751–766

    Google Scholar 

  • Harris, S.I., Balaban, R.S., Barrett, L., Mandel, L.J. 1981. Mitochondrial respiratory capacity and Na+- and K+-dependent adenosine triphosphatase-mediated ion transport in the intact renal cell.J. Biol. Chem. 256:10319–10328

    Google Scholar 

  • Hilden, S.A., Sacktor, B. 1979.d-glucose-dependent sodium transport in renal brush border membrane vesicles.J. Biol. Chem. 254:7090–7096

    PubMed  Google Scholar 

  • Kimmich, G.A., Randles, J. 1981. α-methylglucoside satisfies only Na+-dependent transport system of intestinal epithelium.Am. J. Physiol. 241:C227-C232

    Google Scholar 

  • Kokko, J.P. 1973. Proximal tubule potential difference: Dependence on glucose, HCO3 and amino acids.J. Clin. Invest. 52:1362–1367

    PubMed  Google Scholar 

  • Kolinska, J. 1970. Kinetics of sugar transport in rabbit kidney cortexin vitro: movement ofd-galactose, 2-deoxy-d-galactose and α-methyl-d-glucoside.Biochim. Biophys. Acta 219:200–219

    PubMed  Google Scholar 

  • Mandel, L.J. 1982. Use of noninvasive fluorometry and spectrophotometry to study epithelial metabolism and transport.Fed. Proc. 41:36–41

    PubMed  Google Scholar 

  • Mandel, L.J., Balaban, R.S. 1981. Stoichiometry and coupling of active transport to oxidative metabolism in epithelial tissues.Am. J. Physiol. 240:F357-F371

    PubMed  Google Scholar 

  • McKeown, J.W., Brazy, P.C., Dennis, V.W. 1979. Intrarenal heterogeneity for fluid, phosphate, and glucose absorption in the rabbit.Am. J. Physiol. 237:F312-F318

    PubMed  Google Scholar 

  • Reyonlds, R., Segal, S. 1974. Effects of dibutyryl cyclic AMP on α-methyl-d-glucoside accumulation in rabbit kidney.Am. J. Physiol. 226:791–795

    PubMed  Google Scholar 

  • Segal, S., Rosenhagen, M., Rea, C. 1973. Developmental and other characteristics of α-methyl-d-glucoside transport by rat kidney cortex slices.Biochim. Biophys. Acta 291:519–530

    PubMed  Google Scholar 

  • Silverman, M. 1974a. The chemical and steric determinants governing sugar interactions with renal tubular membranes.Biochim. Biophys. Acta 332:248–262

    Google Scholar 

  • Silverman, M. 1974b. Thein vivo localization of high affinity phlorizin receptors to the brush border surface of the proximal tubule in dog kidney.Biochim. Biophys. Acta 339:92–102

    PubMed  Google Scholar 

  • Silverman, M. 1976. Glucose transport in the kidney.Biochim. Biophys. Acta 457:303–351

    PubMed  Google Scholar 

  • Spring, K. R., Giebisch, G. 1977. Kinetics of Na+ transport inNecturus proximal tubule.J. Gen. Physiol. 70:307–328

    PubMed  Google Scholar 

  • Thierry, J., Poujeol, P., Ripoche, P. 1981. Interactions between Na+-dependent uptake ofd-glucose, phosphate, andl-alanine in rat renal brush border membrane vesicles.Biochim. Biophys. Acta 647:203–210

    PubMed  Google Scholar 

  • Turner, R.J., Moran, A. 1982. Heterogeneity of sodium-dependentd-glucose transport sites along the proximal tubule: Evidence from vesicle studies.Am. J. Physiol. 242:F406-F414

    Google Scholar 

  • Turner, R.J., Silverman, M. 1977. Sugar uptake into normal human renal brush border vesicles.Proc. Natl. Acad. Sci. USA 75:2825–2829

    Google Scholar 

  • Turner, R.J., Silverman, M. 1978. Sugar uptake into brush border vesicles from dog kidney: II. Kinetics.Biochim. Biophys. Acta 511:470–486

    PubMed  Google Scholar 

  • Ullrich, K.J. 1979. Sugar, amino acid, and Na+ cotransport in the proximal tubule.Annu. Rev. Physiol. 41:181–195

    Google Scholar 

  • Vick, H., Diedrich, D.F., Baumann, K. 1973. Re-evaluation of renal tubular glucose transport inhibition by phlorizin analogs.Am. J. Physiol. 224:552–557

    PubMed  Google Scholar 

  • Vinay, P., Gougoux, A., Lemieux, G. 1982. Isolation of a pure suspension of rat proximal tubules.Am. J. Physiol. 241:F403-F411

    Google Scholar 

  • Weidemann, M.J., Krebs, H.A. 1969. The fuel of respiration or rat kidney cortex.Biochem. J. 112:149–166

    PubMed  Google Scholar 

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  1. Steven R. Gullans

    Present address: Department of Physiology, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, CT

Authors and Affiliations

  1. Department of Physiology, Duke University Medical Center, 27710, Durham, North Carolina

    Steven R. Gullans, Stuart I. Harris & Lazaro J. Mandel

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  1. Steven R. Gullans
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  2. Stuart I. Harris
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  3. Lazaro J. Mandel
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Gullans, S.R., Harris, S.I. & Mandel, L.J. Glucose-dependent respiration in suspensions of rabbit cortical tubules. J. Membrain Biol. 78, 257–262 (1984). https://doi.org/10.1007/BF01925973

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  • DOI: https://doi.org/10.1007/BF01925973

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