Summary
Previous investigations of the frequency response of the action of light on the membrane potential inNitella have revealed 5 time constants and 3 zeros in the range of 2 sec to 20 min. Now, the frequency range is extended by means of filter and correlation techniques to its upper theoretical limit of about 20 Hz. Thus the number of experimentally accessible time constants is increased from 5 to 8, the number of the zeros from 3 to 5. The time constant at 0.01 sec is shown to originate from the electrical impedance of the membrane. The other 7 time constants are labels for the reactions mediating the light effect, and the zeros provide information about the arrangement of these reactions. The new results support and extend the basic concept proposed by Hansen (Hansen, U.-P. 1978;J. Membrane Biol. 41: 197) that light acts via three parallel pathways on membrane potential. This model predicts two essential parameters obtained from the high-frequency measurements correctly from the data gained at low frequencies and is capable of reducing the great variety of measured curve shapes of the frequency responses to much smaller changes in two basic parameters.
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References
Bernasconi, C.F. 1976. Relaxation Kinetics. Academic Press, New York-San Francisco-London
Charkewitsch, A.A. 1968. Signale und Störungen. pp. 74–82 and 272–274. Oldenburg-Verlag. Munich-Vienna
Eigen, M. 1968. New looks and outlooks on physical enzymology.Q. Rev. Biophys. 1(1):3
Forsberg, C. 1965. Nutritional studies ofChara in axenic cultures.Physiol. Plant 18:275
Grahn, J. 1978. On-line Messung des 1/f-Rauschens der Membranspannung inNitella mit Hilfe digitaler Filter und Autokorrelationsfunktionen auf einem Mikrocomputer. Diplom-thesis. University of Kiel, Kiel
Hansen, U.-P. 1970. Ein digitaler Sinusgenerator für sehr lange Periodendauern.Elektronik 19:221
Hansen, U.-P. 1974. Preliminary results of an approach to the quantitative description of the action of light on the membrane potential ofNitella.In: Membrane Transport in Plants. U. Zimmermann and J. Dainty, editors. pp. 139–145. Springer-Verlag, Berlin-Heidelberg-New York
Hansen, U.-P. 1978. Do the light-induced changes in the membrane potential ofNitella reflect the feed-back regulation of a cytoplasmic parameter?J. Membrane Biol. 41:197
Hansen, U.-P., Gradmann, D. 1971. The action of sinusoidally modulated light on the membrane potential ofAcetabularia.Plant Cell Physiol. 12:335
Hansen, U.-P., Keunecke, P. 1977. Parallel pathways in the action of light on membrane potential inNitella.In: Transmembrane Ionic Exchanges in Plants. M. Thellier, A. Monnier, M. Demarty, and J. Dainty, editors. pp. 333–340. C.N.R.S., Rouen/Paris
Hansen, U.-P., Warncke, J., Keunecke, P. 1973. Photoelectric effects inCharacean cells. I. The influence of light intensity.Biophysik 9:197
Korff, H.-M. 1979. Untersuchungen zur Analyse eines biologischen Informations-übertragungssystems mit stochastischen Testsignalen. Ph. D. Thesis, University of Kiel, Kiel
Strobel, H. 1968. Systemanalyse mit determinierten Testsignalen. VEB-Verlag Technik, Berlin
Warncke, J. 1974.l/f-Rauschen des Membranpotentials vonNitella und sein Einfluß auf die Lichtantwort.Ber. Dtsch. Bot. Ges. 87:537
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Martens, J., Hansen, UP. & Warncke, J. Further evidence for the parallel pathway model of the metabolic control of the electrogenic pump inNitella as obtained from the high frequency slope of the action of light. J. Membrain Biol. 48, 115–139 (1979). https://doi.org/10.1007/BF01872855
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DOI: https://doi.org/10.1007/BF01872855