Summary
Previous use of hemolysis time measurement to determine permeability coefficients for the red blood cell membrane rested on the assumption that cells swelling in a hypotonic medium hemolyzed immediately on reaching critical volume. By preswelling red cells to various volumes prior to immersion in hemolytic solutions we extrapolate to the hemolysis time of red cells immersed at critical volume and thereby find a significant period of time during which the cells apparently remain in a spherical form prior to release of hemoglobin. Revised estimates of permeability coefficients follow from including this spherical (nonswelling) phase. In addition, the appreciation of a characteristic time period during which the membrane is under tension provides new opportunity to study physical and chemical properties of the membrane.
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References
Altman, P.L. 1961. Biological Handbooks: Blood and Other Body Fluids. p. 19. Federation of American Societies for Experimental Biology, Washington, D.C.
Beck, J.S., Jay, A.W.L., Saari, J.T. 1972. Effects of cytochalasin B on osmotic fragility and deformability of human erythrocytes.Canad. J. Physiol. Pharmacol. 50:684
Cabantchik, Z.I., Rothstein, A. 1974. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation.J. Membrane Biol. 15:207
Caham, P.B., Parkinson, D.R. 1970. The area and volume of single human erythrocytes during gradual osmotic swelling to hemolysis.Canad. J. Physiol. Pharmacol. 48:369
Christiansen, J. 1970. Numerical solution of ordinary simultaneous differential equations of the 1st order using a method for automatic step change.Numer. Math. 14:317
Colley, C.M., Zwaal, R.F.A., Roelofsen, B., van Deenen, L.L.M. 1973. Lytic and non-lytic degradation of phospholipids in mammalian erythrocytes by pure phospholipases.Biochim. Biophys. Acta 307:74
Danon, D. 1961. Osmotic hemolysis by a gradual decrease in the ionic strength of the surrounding medium.J. Cell. Comp. Physiol. 57:111
Dick, D.A.T. 1959. Osmotic properties of living cells.Int. Rev. Cytol. 8:387
Evans, E., Fung, Y.-C. 1972. Improved measurements of erythrocyte geometry.Microvascular Res. 4:335
Fletcher, J.E., Schrager, R.I. 1968. A user's guide to least squares model fitting.NIH Div. Computer Res. Technol., Bethesda, Maryland
Fox, L. 1962. Numerical Solution of Ordinary and Partial Differential Equations. p. 24. Pergamon Press, London
Goldstein, D.A., Solomon, A.K. 1960. Determination of equivalent pore radius for human red cell by osmotic pressure measurement.J. Gen. Physiol. 44:1
Jacobs, M.H. 1952. The measurement of cell permeability with particular reference to the erythrocyte.In: Trends in Physiology and Biochemistry. E.S. Guzman Barron, editor. p. 149. Academic Press, New York
Jay, A.W.L. 1975. Geometry of the human erythrocyte. I. Effect of albumin on cell geometry.Biophys. J. 15:205
Johnson, J.A., Wilson, T.A. 1967. Osmotic volume changes induced by a permeable solute.J. Theoret. Biol. 17:304
Jung, C.Y., Carlson, L.M., Balzer, C.J. 1973. Characteristics of the permeability barrier of human erythrocyte ghosts to non-electrolytes.Biochim. Biophys. Acta 298:101
Kedem, O., Katchalsky, A. 1958. Thermodynamic analysis of the permeability of biological membranes to non-electrolytes.Biochim. Biophys. Acta 27:229
Kendall, M.G., Stuart, A. 1963. The Advanced Theory of Statistics. Vol. 1. Distribution Theory. p. 39. Charles Griffin & Co., London
Kregenow, F.M. 1971. The response of duck erythrocytes to nonhemolytic hypotonic media. Evidence for a volume-controlling mechanism.J. Gen. Physiol. 58:372
Ponder, E. 1971. Hemolysis and Related Phenomena. p. 85. Grune & Stratton, London
Poznansky, M., Solomon, A.K. 1972. Regulation of human red cell volume by linked cation fluxes.J. Membrane Biol. 10:259
Saari, J.T., Beck, J.S. 1974. Probability density function of the red cell membrane permeability coefficient.Biophys. J. 14:33
Schmidt-Ullrich, R., Knüfermann, H., Wallach, D.F.H. 1973. The reaction of 1-dimethyl-aminonaphthalene-5-sulfonyl chloride (DANSCL) with erythrocyte membranes. A new look at “vectorial” membrane probes.Biochim. Biophys. Acta 307:353
Schwoch, G., Passow, H. 1973. Preparation and properties of human erythrocyte ghosts.Mol. Cell. Biochem. 2:197
Seeman, P. 1967. Transient holes in the erythrocyte membrane during hypotonic hemolysis and stable holes in the membrane after lysis by saponin and lysolecithin.J. Cell Biol. 32:55
Seeman, P., Sauks, F., Argent, W., Kwant, W.O. 1969. The effect of membrane-strain rate and of temperature on erythrocyte fragility and critical hemolytic volume.Biochim. Biophys. Acta 183:476
Smith, C.L. 1973. Automatic step change Merson differential equation solver.In: The IMSL Library, Vol. 1, p. DASCRU. International Mathematical and Statistical Libraries, Inc., Houston
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Saari, J.T., Beck, J.S. Hypotonic hemolysis of human red blood cells: a two-phase process. J. Membrain Biol. 23, 213–226 (1975). https://doi.org/10.1007/BF01870251
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DOI: https://doi.org/10.1007/BF01870251