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Na+ and K+ fluxes stimulated by Na+-coupled glucose transport: Evidence for a Ba2+-insensitive K+ efflux pathway in rabbit proximal tubules

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Summary

Addition of glucose or the nonmetabolizable analogue α-methyl-d-glucoside to rabbit proximal tubules suspended in a glucoseand alanine-free buffer caused a sustained increase in intracellular Na+ content (+43±7 nmol · (mg protein)−1) and a concomitant but larger decrease in K+ content (−72±11 nmol· (mg protein)−1). A component of the net K+ efflux was Ba2+ insensitive, and was inhibited by high (1mm) but not low (10 μm) concentrations of the diuretics, furosemide and bumetanide. The increase in intracellular Na+ content is consistent with the view that the increased rates of Na+ and water transport seen in the proximal tubule in the presence of glucose can be attributed (at least in part) to a stimulation of basolateral pump activity by an increased [Na+] i .

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Authors and Affiliations

  1. Departments of Molecular Biophysics & Biochemistry, and Physiology, Yale University School of Medicine, New Haven, Connecticut

    M. J. Avison, S. R. Gullans, T. Ogino & G. Giebisch

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  1. M. J. Avison
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  2. S. R. Gullans
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  3. T. Ogino
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  4. G. Giebisch
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Avison, M.J., Gullans, S.R., Ogino, T. et al. Na+ and K+ fluxes stimulated by Na+-coupled glucose transport: Evidence for a Ba2+-insensitive K+ efflux pathway in rabbit proximal tubules. J. Membrain Biol. 105, 197–205 (1988). https://doi.org/10.1007/BF01870997

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  • DOI: https://doi.org/10.1007/BF01870997

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