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Concentration-dependent modulations of potassium and calcium currents of rat osteoblastic cells by arachidonic acid

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Abstract

We show that the voltage-gated K+ and Ca2+ currents of rat osteoblastic cells are strongly modulated by arachidonic acid (AA), and that these modulations are very sensitive to the AA concentration. At 2 or 3 μm, AA reduces the amplitude and accelerates the inactivation of the K+ current activated by depolarization; at higher concentrations (≥5 μm), AA still blocks this K+ current, but also induces a very large noninactivating K+ current. At 2 or 3 μm, AA enhances the T-type Ca2+ current, close to its threshold of activation, whereas at 10 μm, it blocks that current. AA (1–10 μm) also blocks the dihydropyridine-sensitive L-type Ca2+ current. Thus, the effect of AA on Ca2+ entry through voltage-gated Ca2+ channels can change qualitatively with the AA concentration: at 2 or 3 μm, AA will favor Ca2+ entry through T channels, both by lowering the voltage-gated K+ conductance and by increasing the T current, whereas at 10 μm, AA will prevent Ca2+ entry through voltage-gated Ca2+ channels, both by inducing a K+ conductance and by blocking Ca2+ channels.

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Authors and Affiliations

  1. Laboratoire de Neurobiologie, Ecole Normale Supérieure, 46 rue d'Ulm, 75005, Paris, France

    D. Chesnoy-Marchais

  2. Laboratoire des Tissus Calcifiés, Hôpital des Enfants-Malades, 149 rue de Sèvres, 75015, Paris, France

    J. Fritsch

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  1. D. Chesnoy-Marchais
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  2. J. Fritsch
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Additional information

We wish to thank P. Ascher and J.S. Kehoe for helpful discussion and critical reading of the manuscript. We are very grateful to J. Pons for patient and expert secretarial assistance. This work was supported by the CNRS (URA 295, URA 583).

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Chesnoy-Marchais, D., Fritsch, J. Concentration-dependent modulations of potassium and calcium currents of rat osteoblastic cells by arachidonic acid. J. Membarin Biol. 138, 159–170 (1994). https://doi.org/10.1007/BF00232644

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  • DOI: https://doi.org/10.1007/BF00232644

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