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Adhesion, growth, and matrix production by osteoblasts on collagen substrata

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Summary

A number of studies have demonstrated the pivotal role of collagen molecules in modulating cell growth and differentiation. In order to analyze the direct effects of collagen type I on the osteoblastic phenotype, we have devised an in vitro culture system for studying the interactions between bovine collagen type I and Saos-2 cells, a human osteoblastic cell line. Saos-2 cells were cultured both on top of collagen-coated culture dishes as well as inside a three-dimensional collagen network. Plating on dishes treated with collagen induced maximal adhesion of Saos-2 cells after 24-hour incubation. Cells cultured on collagen gel matrix expressed about 2.5-fold more alkaline phosphatase when compared with untreated plastic dishes. On collagen-coated dishes the responsiveness of Saos-2 cells to parathyroid hormone was decreased, whereas no modifications were observed in the effect of vasoactive intestinal peptide on these cells. Using a microfluorimetric measurement of DNA, an increase of proliferation was observed in Saos-2 cells cultured on collagen gel Saos-2 cells were also able to colonize collagen sponges and in this three-dimensional network they were able to synthesize osteocalcin, as assessed both by immunocytochemistry and radioimmunoassay. In this study we have demonstrated that bovine collagen type I exhibits favorable effects on attachment and functional and growth activities of a human osteoblastic cell line, encouraging its use as a bone graft material.

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Authors and Affiliations

  1. Department of Clinical Physiopathology Endocrine Unit, University of Florence, Viale Pieraccini, 6 50139, Florence, Italy

    L. Masi, V. Giannone, S. Benvenuti & M. L. Brandi

  2. Institute of Morbid Anatomy and Histopathology, University of Florence, Viale Pieraccini, 6 50139, Florence, Italy

    A. Franchi, M. Santucci, D. Danielli & L. Arganini

  3. Department of Human Anatomy and Histology, University of Florence, Viale Pieraccini, 6 50139, Florence, Italy

    L. Formigli

  4. Institute of Internal Medicine III, University of Florence, Viale Pieraccini, 6 50139, Florence, Italy

    A. Tanini

  5. Gentili Institute, Pisa, Italy

    F. Beghe & M. Mian

Authors
  1. L. Masi
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  2. A. Franchi
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  3. M. Santucci
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  4. D. Danielli
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  5. L. Arganini
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  6. V. Giannone
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  7. L. Formigli
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  8. S. Benvenuti
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  10. F. Beghe
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  12. M. L. Brandi
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Masi, L., Franchi, A., Santucci, M. et al. Adhesion, growth, and matrix production by osteoblasts on collagen substrata. Calcif Tissue Int 51, 202–212 (1992). https://doi.org/10.1007/BF00334548

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  • DOI: https://doi.org/10.1007/BF00334548

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