Summary
125I-labeled rat albumin injected intravenously into rats was taken up by growing bone. Some of this radioactive albumin could be removed from bone by washing with saline, the proportion so removed decreasing from 82.5% at 1 day to 7.4% at 8 days. Both the total radioactivity, and that remaining in bone after saline wash, were reduced when growth was slowed by alteration or restriction of the diet. Although the amount of125I albumin in rachitic bone was reduced, autoradiography showed that radioactivity was present in rachitic osteoid. Immunoprecipitation using anti-rat-albumin serum showed that about half of the radioactivity released from normal bone by EDTA was still attached to albumin. These results suggest that albumin plays some part in the growth of bone.
Similar content being viewed by others
References
Ashton, B.A.: Studies on the isolation and characterisation of some macromolecular constituents of bone matrix. D.Phil. Thesis, Oxford University 1972
Bailey, N.T.J.: Statistical methods in biology. p. 98. English Universities Press 1959
Baylink, D., Wergedal, J., Thomson, E.: Loss of protein-polysaccharide at sites where bone mineralization is initiated. J. Histochem. Cytochem.20, 279–292 (1972)
Caster, W.O., Poncelet, J., Simon, A.B., Armstrong, W.D.: Tissue weights of the rat. I. Normal values determined by dissection and chemical methods. Proc. Soc. Exp. Biol. Med.91, 122–126 (1956)
Debro, J.R., Tarver, H., Korner, A.: The determination of serum albumin and globulin by a new method. J. Lab. Clin. Med.50, 728–732 (1957)
Delsal, J.L., Manhouri, H.: Étude comparative des dosages colorimètrique du phosphore: IV. Dosage de l'orthophosphate en presence d'esters phosphoriques (nouvelles méthodes). Bull. Soc. Chim. biol. (Paris)40, 1623–1636 (1958)
DeLuca, H.F., Guroff, G., Steenbock, H., Reiser, S., Marratt, M.R.: Effect of various vitamin deficiencies on citric acid metabolism in the rat. J. Nutr.75, 175–180 (1961)
Herring, G.M.: The organic matrix of bone. In: The biochemistry and physiology of bone, vol, 1, 2nd ed. (Bourne, G.H., ed.) pp. 127–189. New York: Academic Press 1972
Hunter, W.M., Greenwood, F.C.: Preparation of iodide-131 labelled human growth hormone of high specific activity. Nature (London)1974, 495–496 (1962)
Judah, J.D., Nicholls, M.R.: The separation of intracellular serum albumin from rat liver. Biochem. J.123, 643–648 (1971)
Katz, J., Bonorris, G., Golden, S., Sellars, A.L.: Extravascular albumin mass and exchange in rat tissues. Clin Sci.39, 705–724 (1970)
Kirsch, R., Frith, L., Black, E., Hoffenberg, R.: Regulation of albumin synthesis and catabolism by alteration of dietary protein. Nature (London)217, 578–579 (1968)
Lipp, W.: Blood serum proteins and the mineralization of bone ground substance. Histochemie9, 339–353 (1967)
Owen, M., Triffitt, J.T.: Extravascular albumin in bone tissue. J. Physiol.257, 293–307 (1976)
Owen, M., Triffitt, J.T., Melick, R.A.: Albumin in bone. In: Hard tissue growth, repair and remineralization. Ciba Foundation Symposium,11, 263–287. APS, Amsterdam 1973
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bingham, P.J., Melick, R.A. & Mercuri, S.M. The effect of growth retardation and of osteomalacia on the uptake of albumin by bone. Calc. Tis Res. 25, 29–35 (1978). https://doi.org/10.1007/BF02010748
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02010748