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Influence of dichloromethylene diphosphonate (Cl2MDP) and calcitonin on bone resorption, lactate production and phosphatase and pyrophosphatase content of mouse calvaria treated with parathyroid hormonein vitro

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Abstract

The effect of parathyroid hormone, calcitonin and dichloromethylenediphosphonate (Cl2MDP) on bone resorption, lactate production and the content of alkaline and acid phosphatases and alkaline neutral and acid pyrophosphatases in mouse calvaria in culture was investigated. Parathyroid hormone increased all measured variables. Calcitonin prevented the increase in45Ca release and lactate production but not the increase in the various enzymes. The diphosphonate prevented the increase in45Ca release and lactate production but in addition prevented the increase in acid phosphatase and pyrophosphatase.

Résumé

L'effet de la parathormone, de la calcitonine et du dichloromethylenediphosphonate sur la résorption osseuse, la production de lactate et le contenu en phosphatases alcaline et acide aussi bien que celui en pyrophosphatases alcaline, neutre et acide dans une culture de calvaria de souris a été étudié. La parathormone augmente touts ces paramètres. La calcitonine prévient l'augmentation de la libération du45Calcium et du lactate, mais ne prévient pas l'augmentation des différents enzymes. Le diphosphonate lui prévient en plus l'augmentation de la phosphatase et pyrophosphatase acide.

Zusammenfassung

Die Wirkung von Parathormon, Calcitonin und Dichloromethylen-Diphosphonat auf Knockenresorption, Lactatbildung und den Gehalt an alkalischer und saurer Phosphatase sowie an alkalischer, neutraler und saurer Pyrophosphatase in gezüchteten Mäuse-Calvarien wurde untersucht. Parathormon erhöhte alle diese Parameter, Calcitonin verhinderte die Erhöhung von45Calcium-und Lactatfreisetzung, aber nicht die Zunahme der verschiedenen Enzyme. Das Diphosphonat jedoch verhinderte zudem noch die Erhöhung der sauren Phosphatase und Pyrophosphatase.

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Authors and Affiliations

  1. Department of Pathophysiology, University of Bern, Bern

    D. B. Morgan, A. Monod, R. G. G. Russell & H. Fleisch

  2. Laboratory for Experimental Surgery, Davos

    D. B. Morgan, A. Monod, R. G. G. Russell & H. Fleisch

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  1. D. B. Morgan
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  2. A. Monod
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  3. R. G. G. Russell
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  4. H. Fleisch
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Morgan, D.B., Monod, A., Russell, R.G.G. et al. Influence of dichloromethylene diphosphonate (Cl2MDP) and calcitonin on bone resorption, lactate production and phosphatase and pyrophosphatase content of mouse calvaria treated with parathyroid hormonein vitro . Calc. Tis Res. 13, 287–294 (1973). https://doi.org/10.1007/BF02015418

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