Abstract
A procedure for isolating the chromatoid body from the testis of 40-day-old rats was developed. Electron-microscopical analysis indicated that about 70% of the isolated organelles were chromatoid bodies, while the remaining structures corresponded to dense bodies and probably to satellites. Negative staining of the isolated organelles revealed the presence of polysome-like structures in about 20% of the chromatoid bodies suggesting that the polysomes were not due to contamination with cytoplasmic polysomes. Moreover, the presence of RNA in the stroma of the chromatoid body was confirmed by RNAse-gold staining. Preliminary electrophoretic analysis of the RNA extracted from the organelles revealed the presence of a complex population of RNAs including 5.8 and 5 S ribosomal RNAs but no tRNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 10 March 1997 / Accepted: 7 October 1997
Rights and permissions
About this article
Cite this article
Figueroa, J., Burzio, L. Polysome-like structures in the chromatoid body of rat spermatids. Cell Tissue Res 291, 575–579 (1998). https://doi.org/10.1007/s004410051027
Issue Date:
DOI: https://doi.org/10.1007/s004410051027