Summary
We found cells with calcitonin gene-related peptide-like immunoreactivity and with many cored vesicles in the secretory portions of sweat glands of rat foot pads. About 10% of sweat glands contained single immunoreactive cells. The immunoreactive cells were flaskshaped, with a narrow apex facing the glandular lumen and the bulk of the cell body in the basal half of the glandular wall. In the cytoplasm, there were many vesicles, 100–250 nm in diameter, with cores of various electron densities. These cytochemical and cytological characteristics suggest that the immunoreactive cells are homologous to gastrointestinal basal granulated cells.
Similar content being viewed by others
References
Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298:240–244
Bartho L, Lembeck F, Holzer P (1987) Calcitonin gene-related peptide is a potent relaxant of intestinal muscle. Eur J Pharmacol 135:449–451
Brain SD, Tippins JR, Morris HR, MacIntyre I, Williams TJ (1986) Potent vasodilator activity of calcitonin gene-related peptide in human skin. J Invest Dermatol 87:533–536
Fawcett DW (1986) Neuroendocrine cells, paraneurons. In: Fawcett DW (ed) A textbook of histology, 11th edn. WB Saunders Company, Philadelphia, pp 108–110
Fujita T (1977) Concept of paraneurons. Arch Histol Jap 40 [Suppl]:1–12
Fujita T (1981) Paraneuron, its current implications. In: Kanno T (ed) Paraneurons, their features and functions. Excerpta Medica, Amsterdam, pp 3–9
Grude D (1986) The endocrine cells of the digestive system: amines, peptides, and modes of action. Anat Embryol 175:151–162
Lee Y, Takami K, Kawai Y, Girgis S, Hillyard CJ, MacIntyre I, Emson PC, Tohyama M (1985) Distribution of calcitonin gene-related peptide in the rat peripheral nervous system with reference to its coexistence with substance P. Neuroscience 15:1227–1237
Matsuzawa T, Kurosumi K (1963) The ultrastructure, morphogenesis and histochemistry of the sweat glands in the rat foot pads as revealed by electron microscopy. J electron Microsc 12:175–191
Munger BL, Brusilow SW (1971) The histophysiology of rat plantar sweat glands. Anat Rec 169:1–22
Pearse AGE (1986) The diffuse neuroendocrine system: peptide, amines, placodes and the APUD theory. Prog Brain Res 68:25–31
Rosenfeld MG, Mermond J-J, Amara SG, Swanson LW, Sawchenko PE, Rivier J, Vale WW, Evans RM (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature 304:129–135
Somogyi P, Takagi H (1982) A note on the use of picric acid-paraformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunocytochemistry. Neuroscience 7:1779–1783
Sternberger LA (1986) Immunocytochemistry, 3rd edn. Wiley, New York
Wechsler HL, Fisher ER (1968) Eccrine sweat glands of the rat. Response to induced sweating, hypertension, uremia, and alterations of sodium state. Arch Dermatol 97:189–201
Zamboni L, De Martino C (1967) Buffered picric acid-formaldehyde: a new, rapid fixative for electron microscopy. J Cell Biol 35:148A
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ishida-Yamamoto, A., Tohyama, M. Calcitonin gene-related peptide-like immunoreactive cells in the secretory portions of rat sweat glands. Cell Tissue Res 260, 621–623 (1990). https://doi.org/10.1007/BF00297243
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00297243