Summary
The amount and distribution of the lysosomal enzyme acid phosphatase in light- and dark-adapted eyes of the brackish-water annelid Nereis limnicola were studied by standard cytochemical techniques. Precipitate from the acid phosphatase reaction was observed in Golgi-endoplasmic reticulum-lysosomal complexes, primary lysosomes, and secondary lysosomes, formed by fusion of primary lysosomes with phagocytic and pinocytic vesicles containing products of presumed rhabdomeric degradation. The acid phosphatase reaction occurred in these organelles in both sensory and supportive cells of both light- and darkadapted ocelli. Secondary lysosomes were more abundant in sensory cells of illuminated ocelli than in those maintained in the dark. Sparse reaction product was found in Golgi cisternae, none in rough endoplasmic reticulum. We suggest that the increase of lysosomal activity in light-adapted eyes is correlated with the breakdown of photosensory microvilli upon exposure to light. A diagram of our interpretation of recycling of photoreceptoral membrane in N. limnicola is presented.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barka T, Anderson PJ (1962) Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler. J Histochem Cytochem 10:741–753
Barlow RB, Chamberlain SC (1980) Light and a circadian clock modulate structure and function in Limulus photoreceptors. In: Williams TP, Baker BN (eds) The effects of constant light on visual processes. Plenum, New York, pp 247–269
Barlow RB, Chamberlain SC, Kass L (1984) Circadian rhythms in retinal function. In: Hilfer SR, Sheffield JB (eds) Molecular and cellular basis of visual acuity. Springer, New York, pp 31–53
Blest AD (1980) Photoreceptor membrane turnover in arthropods: comparative studies of breakdown processes and their implications. In: Williams TP, Baker BN (eds) The effects of constant light on visual processes. Plenum, New York, pp 217–245
Blest AD, Day WA (1978) The rhabdomere organization of some nocturnal pisaurid spiders in light and darkness. Philos Trans R Soc Lond (Biol) 283:1–23
Blest AD, Price GD, Maples J (1979) Photoreceptor membrane breakdown in the spider Dinopis: Localisation of acid phosphatases. Cell Tissue Res 199:455–472
Blest AD, Stowe S, Eddey W (1982a) A labile, Ca2+-dependent cytoskeleton in rhabdomeral microvilli of blowflies. Cell Tissue Res 223:553–573
Blest AD, Stowe S, Eddey W, Williams DS (1982b) The local deletion of a microvillar cytoskeleton from photoreceptors of tipulid flies during membrane turnover. Proc R Soc Lond (Biol) 215:469–479
Bocquet M (1977) Etude ultrastructurale de l'organe photorécepteur A'Odontosyllis ctenostoma S/F: Eusyllinae (Annélide Polychète). J Ultrastruct Res 58:210–217
Brandenburger JL (1977) Cytochemical localization of acid phosphatase in regenerated and dark-adapted eyes of a snail, Helix asper sa. Cell Tissue Res 184:301–313
Brandenburger JL, Eakin RM (1980) Cytochemical localization of acid phosphatase in ocelli of the seastar Patiria miniata during recycling of photoreceptoral membranes. J Exp Zool 214:127–140
Couet HG de, Blest AD (1982) The retinal acid phosphatase of a crab, Leptograpsus: Characterisation and relation to the cyclical turnover of photoreceptor membrane. J Comp Physiol 149:353–362
Eakin RM (1965) Differentiation of rods and cones in total darkness. J Cell Biol 25:162–165
Eakin RM, Brandenburger JL (1982) Pinocytosis in eyes of a snail, Helix aspersa. J Ultrastruct Res 80:214–229
Eakin RM, Brandenburger JL (1985) Effects of light and dark on photoreceptors in the polychaete annelid Nereis limnicola. Cell Tissue Res 242:613–622
Eguchi E, Waterman TH (1976) Freeze-etch and histochemical evidence for cycling in crayfish photoreceptor membranes. Cell Tissue Res 169:419–434
Fournier A (1984) Photoreceptors and photosensitivity in platyhelminthes. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum, New York pp 217–239
Hafner GS, Bok D (1977) The distribution of 3H-leucine labeled protein in the retinula cells of the crayfish retina. J Comp Neurol 174:397–416
Hafner GS, Hammond-Soltis G, Tokarski T (1980) Diurnal changes of lysosome-related bodies in the crayfish photoreceptor cells. Cell Tissue Res 206:319–332
Herman KG (1983) Rods, rhabdoms, and rhythms. Bio Science 33:432–438
Horridge GA, Duniec J, Marčelja L (1981) A 24-hour cycle in single locust and mantis photoreceptors. J Exp Biol 91:307–322
Itaya SK (1976) Rhabdom changes in the shrimp, Palaemonetes. Cell Tissue Res 166:265–273
Karnovsky MJ, Robinson JM, Briggs RT, Karnovsky ML (1981) Oxidative cytochemistry in phagocytosis: the interface between structure and function. Histochem J 13:1–22
Kataoka S, Yamamoto TY (1983) Fine structure and formation of the photoreceptor in Octopus ocellatus. Biol Cell 49:45–54
Kataoka S, Yamamoto TY, Tonosaki A, Washioka H (1982) Ultrastructural study of photoreceptor membrane turnover in an invertebrate retina. In: Hollyfield JG (ed) The structure of the eye. Elsevier, New York, pp 35–44
Meinecke C, Langer H (1984) Localization of visual pigments within rhabdoms of the compound eye of Spodoptera exempta (Insecta, Noctuidae). Cell Tissue Res 238:359–368
Nichols BA (1981) Use of ultrastructural histochemistry. In: Adams DO, Edelson PJ, Koren HS (eds) Methods for studying mononuclear phagocytes. Academic Press, New York, pp 413–432
Novikoff AB (1964) GERL, its form and function in neurons of rat spinal ganglia. Biol Bull 127:358
Novikoff AB (1976) The endoplasmic reticulum: A cytochemist's view (A review). Proc Natl Acad Sci USA 73:2781–87
Robinson JM (1984) Improvements in the cerium method for the EM cytochemical localization of phosphatases in single cells: use of detergents. J Cell Biol 99:57a
Robinson JM, Karnovsky MJ (1983) Ultrastructural localization of several phosphatases with cerium. J Histochem Cytochem 31:1197–1208
Robles LJ, Cabebe CS, Aguilo JA, Anyakora PA, Bok D (1984) Autoradiographic and biochemical analysis of photoreceptor membrane renewal in Octopus retina. J Neurocytol 13:145–164
Röhlich P, Tar E (1968) The effect of prolonged light-deprivation on the fine structure of planarian photoreceptors. Z Zellforsch 90:507–518
Schwemer J, Henning U (1984) Morphological correlates of visual pigment turnover in photoreceptors of the fly, Calliphora erythrocephala. Cell Tissue Res 236:293–303
Stowe S (1981) Effects of illumination changes on rhabdom synthesis in a crab. J Comp Physiol 142:19–25
Stowe S (1983) Phagocytosis of rhabdomeral membrane by crab photoreceptors (Leptograpsus variegatus). Cell Tissue Res 234:463–467
Waterman TH (1982) Fine structure and turnover of photoreceptor membranes. In: Westfall JA (ed) Visual cells in evolution. Raven, New York, pp 23–41
White RH (1968) The effect of light and light deprivation upon the ultrastructure of the larval mosquito eye. III. Multivesicular bodies and protein uptake. J Exp Zool 169:261–278
White RH, Gifford D, Michaud NA (1980) Turnover of photoreceptor membrane in the larval mosquito ocellus: rhabdomeric coated vesicles and organelles of the vacuolar system. In: Williams TP, Baker BN (eds) The effects of constant light on visual processes. Plenum, New York, pp 271–296
Whittle AC (1976) Reticular specializations in photoreceptors: a review. Zool Scripta 5:191–206
Williams DS (1982) Ommatidial structure in relation to turnover of photoreceptor membrane in the locust. Cell Tissue Res 225:595–617
Williams DS, Blest AD (1980) Extracellular shedding of photoreceptor membrane in the open rhabdom of a tipulid fly. Cell Tissue Res 205:423–438
Yamamoto M, Yoshida M (1984) Reexamination of the ultrastructure of rhabdomeric microvilli in the Octopus photoreceptor. Biol Cell 52:83–86
Yoshida M, Takasu N, Tamotsu S (1984) Photoreception in echinoderms. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum, New York, pp 743–771
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brandenburger, J.L., Eakin, R.M. Cytochemical localization of acid phosphatase in light- and dark-adapted eyes of a polychaete worm, Nereis limnicola . Cell Tissue Res. 242, 623–628 (1985). https://doi.org/10.1007/BF00225428
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225428