Summary
In a circumscribed area of the preoptic periventricular nucleus of a male rat, formaldehyde-induced monoamine fluorophores modified by treatment with HCl vapors were investigated microfluorometrically (measurement of excitation peak ratio 370∶320 nm) in all fluorescent terminals and preterminals. Microfluorometric recordings of an individual fluorescent structure were performed without UV irradiation of neighboring fluorophores. Recorded data were sampled and corrected by a microcomputer (WangPCS II). 19 neuronal processes (axons) contained noradrenaline fluorophores; 11 contained dopamine fluorophores; 6 exhibited uncharacteristic excitation peak ratios; and in 9 recordings technical problems did not allow identification of the fluorophore content.
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References
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Supported by grants from the Deutsche Forschungsgemeinschaft (-Ha 726/5-) and the Stiftung Volkswagenwerk (1/35582) to H.G.H.
In partial fulfillment of the requirements for an academic degree (Dr. med. dent.)
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Hartwig, H.G., Reinhold, C. & Lyncker, I. Distribution patterns of formaldehyde-induced fluorescence of hypothalamic monoamines. Cell Tissue Res. 201, 499–502 (1979). https://doi.org/10.1007/BF00237008
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DOI: https://doi.org/10.1007/BF00237008