Summary
Using specific antibodies against β subunits of porcine LH and FSH, the pituitary cells which produce these two gonadotrophins were identified in the porcine pituitary at the ultrastructural level using the immunoperoxidase technique. It was clearly shown that most of the gonadotrophic cells are responsible for the production of both FSH and LH. However, some cells, only positive for FSH or LH, indicate that the concentrations of FSH and LH vary from cell to cell. At the ultrastructural level, the FSH/LH cells contain one class of secretory granules strongly positive for both FSH and LH as well as large negatively stained dense bodies. These findings indicate that the “one cell-one hormone” concept may not apply to gonadotrophic hormones; a FSH/LH cell cannot be distinguished from a LH or a FSH cell without immunocytochemical identification of its hormonal content.
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Abbreviations
- p-LH:
-
porcine luteinizing hormone
- p-LHα :
-
porcine LHα subunit
- p-LHβ :
-
porcine LHβ subunit
- p-FSH:
-
porcine follicule stimulating hormone
- p-FSHβ :
-
porcine FSHβ subunit
- b-TSH:
-
bovine thyrotropic hormone
References
Barnes, B.G.: Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinol. 71, 618–628 (1962).
Barnes, B.G.: The fine Structure of the mouse adenohypophysis in various physiological states. In: Cytologie de l'adénohypophyse (J. Benoit and da Lage, ed.). Paris: Editions du Centre National de la Recherche Scientifique 1963
Beauvillain, J.C., Tramu, G., Dubois, M.P.: Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in Lerot pituitary (Eliomys quercinus). Cell Tiss. Res. 158, 301–317 (1975)
Bugnon, C., Lenys, D., Kerdelhué, B., Dessy, C., Fellmann, D.: Etude cytoimmunologique des cellules gonadotropes du Renard roux. C.R. Soc. Biol. (Paris) 168, 814–818 (1974)
Closset, J., Hennen, G. : Porcine follitropin. Isolation and characterization of the native hormone and its α and β subunits. Europ. J. Biochem., in Press (1978)
Dacheux, F., Dubois, M.P.: Ultrastructural localization of prolactin growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary. Cell Tiss. Res. 174, 245–260 (1976a)
Dacheux, F., Dubois, M.P.: Immunocytologie ultrastructurale des cellules à prolactine et des cellules somatotropes et gonadotropes de l'hypophyse de bovins. J. Physiol. (Paris) 72 (sn5), 38A (1976b)
Dacheux, F., Dubois, M.P.: Etude comparée de l'immunoréactivité des cellules à prolactine et des cellules gonadotropes LH de l'hypophyse de béliers et de bovins. J. Physiol. (Paris) 72 (6), 9B (1976c)
Dacheux, F., Dubois, M.P.: Ultrastructural localization of LH, LHα and LHβ in the rat pituitary using the immunoperoxidase technique. IRCS Med. Sci. 5, 580–581 (1977)
Dacheux, F., Dubois, M.P.: The LH-producing cells in the ovine pituitary: an electron microscopic immunocytochemical study. Cell Tiss. Res., in press (1978)
El Etreby, M.F., Fath El Bab, M.R.: Localization of gonadotrophic hormones in the dog pituitary gland. Cell Tiss. Res. 183, 167–175 (1977)
Farquhar, M.G., Rinehart, J.F.: Electron microscopic studies of the anterior pituitary gland of castrate rats. Endocrinol. 54, 516–541 (1954)
Herbert, D.C.: Localization of antisera to LHβ and FSHβ in the rat pituitary gland. Amer. J. Anat. 144, 379–385 (1975)
Herbert, D.C.: Immunocytochemical evidence that luteinizing hormone (LH) and follicle stimulating hormone (FSH) are present in the same cell type in the rhesus monkey pituitary gland. endocrinol. 98, 1554–1557 (1976)
Herlant, M.: Ultrastructure des cellules gonadotropes de l'hypophyse chez les mammifères. In: Hormones glycoprotéïques hypophysaires (M. Jutisz, ed.), pp. 5–29. Paris: Editors I.N.S.E.R.M. 1972
Herlant, M., Ectors, F.: Les cellules gonadotropes de l'hypophyse chez le porc. Z. Zellforsch. 101, 212–231 (1969)
Kurosumi, K., Oota, Y.: Electron microscopy of two types of gonadotrophs in the anterior pituitary gland of persistent oestrus and persistent dioestrus rats. Z. Zellforsch. 85, 34–46 (1968)
Li, J.Y., Dubois, M.P., Dubois, P.M.: Somatotrophs in the human fetal anterior pituitary. An electron microscopic-immunocytochemical study. Cell Tiss. Res. 181, 545–552 (1977)
Mirecka, J., Pearse, A.G.E.: Localization of FSH and LH-producing cells in the pig adenohypophysis by an immunohistochemical technique. Folia histochem. cytochem. 9, 365–371 (1971)
Moriarty, G.C.: Adenohypophysis: ultrastructural cytochemistry. A review. J. Histochem. Cytochem. 21, 855–894 (1973b)
Moriarty, G.C.: Electron microscopic-immunocytochemical studies of rat pituitary gonadotrophs: a sex difference in morphology and cytochemistry of LH cells. Endocrinol. 97, 1215–1225 (1975)
Moriarty, G.C.: Immunocytochemistry of the pituitary glycoprotein hormones. J. Histochem. Cytochem. 24, (7), 846–863 (1976)
Moriarty, G.C., Garner, L.L.: Immunocytochemical studies of cells in the rat adenohypophysis containing both ACTH and FSH. Nature (Lond.) 265, 5592, 356–358 (1977)
Moriarty, G.C., Halmi, N.S.: Adrenocorticotropin production by the intermediate lobe of the rat pituitary. An electron microscopic-immunohistochemical study. Z. Zellforsch. 132, 1–14 (1972)
Moriarty, G.C., Moriarty, C.M., Sternberger, L.A.: Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem. 21, 825–833 (1973a)
Nakane, P.K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)
Nakane, P.K.: Application of peroxidase-labeled antibodies to the intracellular localization of hormones. Acta endocr. (Kbh.), (Suppl.) 153, 190–202 (1971)
Nakane, P.K.: Identification of anterior pituitary cells by immunoelectron microscopy. In: The anterior pituitary (A. Tixier-Vidal and M.G. Farquhar, eds.), pp. 45–61. New York: Acad. Press 1975
Pelletier, G., Leclerc, R., Labrie, F.: Identification of gonadotropic cells in the human pituitary by immunoperoxidase technique. Molec. cell. Endocrinol. 6, 123–126 (1976)
Petrali, J.P., Hinton, D.M., Moriarty, G.C., Sternberger, L.A.: The unlabeled antibody enzyme method of immunocytochemistry. Quantitative comparison of sensitivities with and without peroxidase complex. J. Histochem. Cytochem. 22, 782–801 (1974)
Phifer, R.F., Midgley, A.R., Spicer, S.S.: Immunohistologic and histologic evidence that follicle-stimulating hormone and luteinizing hormone are present in the same cell type in the human pars distalis. J. clin. Endocr. 36, 125–141 (1973)
Rennels, E.G.: Gonadotrophic cells of rat hypophysis: In Cytologie de l'adénohypophyse (Coll. Intern. CNRS n∘ 128, Paris). J. Benoit and C. Da Lage (Edit.) 201–209 (1963)
Rennels, E.G., Bogdanove, E.M., Arimura, A., Saito, M., Schally, A.V.: Ultrastructural observations of rat pituitary gonadotrophs following injection of purified porcine LH-RH. Endocrinol. 88, 1318–1326 (1971)
Robyn, C., Leleux, P., Vanhaelst, L., Golstein, J., Herlant, M., Pasteels, J.L.: Immunohistochemical study of the human pituitary with anti-luteinizing hormone, anti-follicle stimulating hormone and anti-thyrotrophin sera. Acta endocr. (Kbh.) 72, 625–642 (1973)
Stefanini, M., Martino, C. de, Zamboni, L.: Fixation of ejaculated spermatozoa for an electron microscopy. Nature (Lond.) 216, 173–174 (1967)
Tixier-Vidal, A., Tougard, C., Kerdelhué, B., Jutisz, M.: Light and electron microscopic studies on immunocytochemical localization of gonadotropic hormones in the rat pituitary gland with antisera against ovine FSH, LH, LHα and LHβ. Ann. N.Y. Acad. Sci. 254, 433–461 (1975)
Tougard, C., Kerdelhué, B., Tixier-Vidal, A., Jutisz, M.: Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase labeled antibody technique. J. Cell Biol. 58, 503–521 (1973)
Yoshimura, F., Harumiya, K.: Electron microscopy of anterior lobe of pituitary in normal and castrated rats. Endocr. jap. 12, 119–152 (1965)
Zamboni, L., Martino, C. de: Buffered picric acid formaldehyde: a new rapid fixation for electron microscopy. J. Cell Biol. 35, 148A (1967)
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Dacheux, F. Ultrastructural localization of gonadotrophic hormones in the porcine pituitary using the immunoperoxidase technique. Cell Tissue Res. 191, 219–232 (1978). https://doi.org/10.1007/BF00222421
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DOI: https://doi.org/10.1007/BF00222421