Summary
In untreated, fasting animals the cells of the serous demilunes of the sublingual gland incorporate [3H]-leucine at a higher rate than any other of the 5 main cell types of the 3 major salivary glands. The acinar cells of the submandibular and the mucous cells of the sublingual gland show intermediate values, while the cells of the granular ducts of the submandibular and the acini of the parotid gland have a low rate of incorporation.
In fasting animals extrusion of newly synthesized protein starts early in the cells of the serous demilunes. It starts between 4 and 7 hrs after [3H]-leucine injection in the acinar cells of the submandibular gland, while the other cell types did not lose substantial amounts of labelled (glyco)protein within 7 hrs.
The secretion of protein is stimulated by the cholinergic drug pilocarpine in all but one of the 5 types of salivary gland cells studied. The acinar cells of the submandibular gland react strongly, the granular duct cells less strongly. Still less are the reactions of the acinar cells of the parotid and of the mucous cells of the sublingual gland. The cells of the serous demilunes of the latter appear to be insensible to pilocarpine. The effect of food uptake on secretion does not differ from pilocarpine stimulation, with one exception: the acinar cells of the parotid gland react more strongly on food uptake than on cholinergic stimulation.
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References
Barka, T.: Stimulation of protein and ribonucleic acid synthesis in rat submaxillary gland by isoproterenol. Lab. Invest. 18, 38–41 (1968)
Castle, J.D., Jamieson, J.D., Palade, G.E.: Radioautographic analysis of the secretory process in the parotid acinar cell of the rabbit. J. Cell. Biol. 53, 290–311 (1972)
Cutler, L.S., Chaudry, A.P.: Release and restoration of the secretory granules in the convoluted granular tubules of the rat submandibular gland. Anat. Rec. 176, 405–420 (1973)
Enomoto, S., Scott, B.L.: Intracellular distribution of mucosubstances in the major sublingual gland of the rat. Anat. Rec. 169, 71–96 (1971)
Grometh-Elhanan, Z., Winnick, T.: Microsomes as sites of α-amylase synthesis in the rat parotid gland. Biochim. biophys. Acta (Amst.) 69, 85–96 (1963)
Groot, E.C.B.M. de: The relation between synthesis and secretion of pepsinogen. Thesis, Utrecht (1974)
Hanks, C.T., Chaudry, A.P.: Radioautographic evidence of [3H]-tryptophan incorporation in secretory cells of rat submandibular glands. J. dent. Res. 50, 1626–1630 (1971)
Harrop, T.J., Garrett, J.R.: Effects of preganglionic sympathectomy on secretory changes in parotid acinar cells of rats on eating. Cell Tiss. Res. 154, 135–150 (1974)
Heins, P., Tamarin, A.: Response of the rat submaxillary gland to pilocarpine: a quantitative analysis of gross and microscopic changes. J. dent. Res. 47, 575–579 (1968)
Herzog, V., Miller, F.: Die Lokalisation endogener Peroxydase in der Glandula parotis der Ratte. Z. Zellforsch. 107, 403–420 (1970)
Hollmann, K.H., Verley, J.H.: La glande sous-maxillaire de la souris et du rat. Z. Zellforsch. 68, 363–388 (1965)
Kim, S.K., Nasjleti, C.E., Han, S.S.: The secretion process in mucous and serous secretory cells of the rat sublingual gland. J. Ultrastruct. Res. 38, 371–389 (1972)
Kramer, M.F., Poort, C.: Protein synthesis in the pancreas of the rat after stimulation of secretion. Z. Zellforsch. 86, 475–486 (1968)
Luzzatto, A.C., Procicchiani, G., Rosati, G.: Rat submaxillary gland: an electron microscope study of the secretory granules of the acinus. J. Ultrastruct. Res. 22, 185–194 (1968)
Materazzi, G., Vitaioli, L.: Observations on the formation of secretion by the cells of the “convoluted granular tubules” of the submandibular gland of the rat. J. Anat. 105, 163–170 (1969)
Matthews, R.W.: Measurement of protein synthesis in the rat submandibular gland using tritiated tryptophane. Arch. Oral Biol. 19, 985–988 (1974a)
Matthews, R.W.: The effects of autonomic stimulation upon the rat submandibular gland. Arch. Oral Biol. 19, 989–994 (1974b)
Poort, C., Kramer, M.F.: Effect of feeding on the protein synthesis in mammalian pancreas. Gastroenterology 57, 689–696 (1969)
Robinovitch, M.R., Sreebny, L.N., Smudder, E.A.: Ribonuclease and ribonuclease inhibitor of the rat parotid gland and its secretion. J. biol. Chem. 243, 3441–3446 (1968)
Scott, B.L., Pease, D.C.: Electron microscopy of induced changes in the salivary gland of the rat. In: Salivary glands and their secretions (L.N. Sreebny and J. Meyer, eds.). New York: MacMillan, 1964
Shear, M.: Histological and histochemical studies of intercalated ducts in rat parotid glands. S. Air. med. J. 33, 97–103 (1968)
Shear, M.: Substrate film techniques for the histochemical demonstration of amylase and protease in salivary glands. J. dent. Res. 51, 368–380 (1972)
Sreebny, L.N., Johnson, D.A., Robinovitch, M.R.: Functional regulation of protein synthesis in the rat parotid gland. J. biol. Chem. 246, 3879–3884 (1971)
Tiber, A.: Radioautographic and histochemical study of protein synthesis in the hamster submandibular gland. J. dent. Res. 50, 837–845 (1971)
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Kuijper-Lenstra, A.H., Kramer, M.F. Rate of protein synthesis in rat salivary gland cells after pilocarpine or feeding. Cell Tissue Res. 164, 435–446 (1975). https://doi.org/10.1007/BF00219935
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DOI: https://doi.org/10.1007/BF00219935