Summary
A previously described genomic library constructed from microdissected DNA has been used to generate a large number of probes around the adenomatous polyposis coli (APC) gene at 5q22. A total of 202 clones were hybridised directly onto a somatic cell hybrid panel containing two APC-related interstitial deletions. Of 75 microclones that gave clear hybridisation signals, 22 independent clones mapped into the region common to both deletions. In addition, 4/22 of the markers are conserved in rodent DNA. These clones should provide a valuable resource for screening cDNA libraries and cloning the DNA around the APC gene in yeast artificial chromosomes.
This is a preview of subscription content, log in via an institution to check access.
References
Bodmer WF, Bailey CJ, Bodmer J, Bussey HJR, Ellis A, Gorman P, Lucibello FC, Murday VA, Rider SH, Scambler P, Sheer D, Solomon E, Spurr NK (1987) Localization of the gene for familial adenomatous polyposis on chromosome 5. Nature 328:614–616
Buiting K, Neumann M, Lüdecke H-J, Senger G, Claussen U, Antich J, Passarge E, Horsthemke B (1990) Microdissection of the Prader-Willi syndrome chromosome region and identification of potential gene sequences. Genomics 6:521–527
Bussey HJR (1975) Familial polyposis coli. Family studies, histopathology, differential diagnosis, and results of treatment. Johns Hopkins University Press, Baltimore London
Cotter F, Hampton GM, Nasipuri S, Bodmer WF, Young BD (1990) Rapid isolation of human chromosome-specific probes from a somatic cell hybrid. Genomics 7:257–263
Coulson A, Sulston J, Brenner S, Karn J (1986) Towards a physical map of the genome of the nematode Ceanorhabditis elegans. Proc Natl Acad Sci USA 83:7821–7825
Fearon ER, Vogelstein B (1990) A genetic model for colorectal tumorigenesis. Cell 61:759–767
Feinberg A, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Anal Biochem 132:6–13
Hampton GM, Leuteritz G, Lüdecke H-J, Senger G, Trautmann U, Thomas H, Solomon E, Bodmer WF, Horsthemke B, Claussen U, Ballhausen WG (1991) Characterisation and mapping of microdissected genomic clones to the adenomatous polyposis coli (APC)-region. Genomics (in press)
ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Karger, Basel
Kinzler KW, Nilbert MC, Vogelstein B, Bryan TM, Levy DB, Smith KJ, Preisinger AC, Hamilton SR, Hedge P, Markham A, Carlson M, Joslyn G, Groden J, White R, Miki Y, Miyoshi Y, Nishisho I, Nakamura Y (1991) Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers. Science 251:1366–1370
Larin Z, Monaco AP, Lehrach H (1991) Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc Natl Acad Sci USA 88:4123–4127
Leppert M, Dobbs M, Scambler P, O'Connell P, Nakamura Y, Stauffer D, Woodward S, Burt R, Hughes J, Gardner E, Lathrop M, Wasmuth J, Lalouel J-M, White R (1987) The gene for familial polyposis coli maps to the long arm of chromosome 5. Science 238:1411–1413
MacKinnon RN, Hirst MC, Bell MV, Watson JEV, Claussen U, Lüdecke HJ, Senger G, Horsthemke B, Davies KE (1990) Microdissection of the fragile X region. Am J Hum Genet 47:181–187
Nakamura Y, Lathrop M, Leppert M, Dobbs M, Wasmuth J, Wolff E, Carlson M, Fujimoto E, Krapcho K, Sears T, Woodward S, Hughes J, Burt R, Gardner E, Lalouel J-M, White R (1988) Localization of the genetic defect in familial adenomatous polyposis within a small region of chromosome 5. Am J Hum Genet 43:638–644
Solomon E (1990) Colorectal cancer genes. Nature 343:412–414
Trautmann U, Leuteritz G, Senger G, Claussen U, Ballhausen WG (1991) Detection of APC region specific signals by non-isotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe. Hum Genet 87:495–497
Varesco L, Thomas HJ, Williams S, Fennell SJ, Hockey A, Searle S, Bodmer WF, Frischauf A-M, Solomon E (1989) CpG island clones from a deletion encompassing the gene for adenomatous polyposis coli. Proc Natl Acad Sci USA 86:10118–10122
Williams SV, Jones TA, Cottrell S, Zehetner G, Varesco L, Ward T, Thomas H, Lawson PA, Solomon E, Bodmer WF, Frischauf A-M, Sheer D (1991) Fine mapping of probes in the adenomatous polyposis coli region of chromosome 5 by in situ hybridisation. Genes Chromosomes Cancer (in press)
Author information
Authors and Affiliations
Additional information
This work will be presented in part at HGM11, London.
Rights and permissions
About this article
Cite this article
Hampton, G.M., Howe, C., Leuteritz, G. et al. Regional mapping of 22 microclones around the adenomatous polyposis coli (APC) locus on chromosome 5q. Hum Genet 88, 112–114 (1991). https://doi.org/10.1007/BF00204940
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00204940