Summary
Human × rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14–q15. M6P1 is distinct from NT5, coding for 5′ nucleotidase, which maps to the same region.
Similar content being viewed by others
References
Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A (1978) Production of monoclonal antibodies of group A erythrocytes, HLA and other human surface antigens. Cell 14:9–20
Bosch R van den, Geuze HJ, Maine APM du, Strous GJ (1986) Transport and metabolism of 5′-nucleotidase in a rat hepatoma cell line. Eur J Biochem 160:49–54
Boyle JM, Hey Y, Geurts van Kessel A, Fox M (1988) Assignment of ecto-5′ nucleotidase to human chromosome 6. Hum Genet 81:88–92
Boyle JM, Hey Y, Fox M (1989a) Nucleotide ecto-enzyme activities of human and Chinese hamster fibroblasts in tissue culture. Biochem Genet 27:655–671
Boyle JM, Hey Y, Grzeschik K-H, Thompson L, Munro E, Fox M (1989b) Regional localization of human ecto-5′ nucleotidase to chromosome 6q14–q21. Hum Genet 83:179–180
De Bruyn CHMM, Simmonds HA, Muller MM (eds) (1984) Purine metabolism in man — IV. Adv Exp Med Biol 165
Grzeschik K-H, Mayerova A, Bender K, Johannsmann R (1978) Regional submapping of human chromosome 6 by the use of human-mouse somatic cell hybrids. Cytogenet Cell Genet 22:412–414
Hole N, Stern PL (1988) A 72 kD trophoblast glycoprotein defined by a monoclonal antibody. Br J Cancer 57:239–246
Hole N, Stern PL (1990) Isolation and characterization of 5T4, a tumour associated antigen. Int J Cancer 45:179–184
Jones H, Roberts G, Hole M, McDicken IW, Stern PL (1990) Investigation of expression of 5T4 antigen in cervical cancer. Br J Cancer 61:96–100
Low MG, Finean JB (1978) Specific release of plasma membrane enzymes by a phosphatidylinositol-specific phospholipase C. Biochim Biophys Acta 508:565–570
Shows TB, McAlpine PJ, Boucheix C, et al (1987) Guidelines for human gene nomenclature. (9th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 46:11–28
Southall PJ, Boxer GM, Bagshawe KD, Hole N, Stern PL (1990) Immunohistological distribution of 5T4 antigen in normal and malignant tissues. Br J Cancer 61:89–95
Stern PL, Gilbert P, Sternberg S, Thompson S, Chada K (1984) A monoclonal antibody which detects a 125 kDa glycoprotein on embryonal carcinoma cells and is mitogenic for murine spleen cells. J Reprod Immunol 6:313–328
Thompson LF, Ruedi JM, Low MG (1987) Purification of 5′ nucleotidase from human placenta after release from plasma membrane by phosphatidylinositol-specific phospholipase C. Biochim Biophys Acta 145:118–125
Wake N, Hreshchyshyn MM, Piver SM, Matsui S, Sandberg AA (1980) Specific cytogenetic changes in ovarian cancer involving chromosome 6 and 14. Cancer Res 40:4512–4518
Wild CP, Smart GM, Saffhill R, Boyle JM (1983) Radioimmunoassay of O6-methyldeoxyguanosine in DNA of cells alkylated in vitro. Carcinogenesis 4:1605–1609
Winquist R, Turunen O, Pakkanon R, Grzeschik K-H, Wahlstrom T, Vaheri A (1989) Localization of the cytovillin gene to region q22–q27 of human chromosome 6 (abstract). (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:1108–1109
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Boyle, J.M., Grzeschik, K.H., Heath, P.R. et al. Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14–q15. Hum Genet 84, 455–458 (1990). https://doi.org/10.1007/BF00195819
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00195819